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Detecting Glycogen in Peripheral Blood Mononuclear Cells with Periodic Acid Schiff Staining
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Immunology and Infection
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JoVE Journal Immunology and Infection
Detecting Glycogen in Peripheral Blood Mononuclear Cells with Periodic Acid Schiff Staining

Detecting Glycogen in Peripheral Blood Mononuclear Cells with Periodic Acid Schiff Staining

DOI:
10.3791/52199-v

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09:42 min

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December 23, 2014

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, , , , ,
1Department of Biology,Centre for Structural and Functional Genomics, PERFORM Centre, Concordia University, 2Department of Chemistry and Biochemistry,Centre for Structural and Functional Genomics, PERFORM Centre, Concordia University, 3Department of Exercise Science,Centre for Structural and Functional Genomics, PERFORM Centre, Concordia University

Chapters

  • 00:05Title
  • 01:34PBMC Isolation from Whole Blood
  • 05:15Making the PBMC Slide and Fixing the Samples
  • 06:13Making the Amylase Solution for Negative Control
  • 07:00Perform Periodic Acid Schiff (PAS) Staining and Imaging
  • 08:11Results: PAS-staining on Human PBMCs
  • 09:17Conclusion

Summary

Automatic Translation

Automatic Translation

Periodic acid Schiff staining is a technique that visualizes the polysaccharide content of tissues. This article demonstrates periodic acid Schiff staining protocol adapted for use on peripheral blood mononuclear cells purified from human venous blood. Such samples are enriched for lymphocytes and other white blood cells of the immune system.

Tags

Keywords: PAS StainingGlycogenPeripheral Blood Mononuclear CellsImmunohistochemistryLymphocytesGlycoproteinsGlycolipidsMuscle CellsImmune SystemEnergy SourceAmylaseEnzymatic Digestion

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