Name: Generation and Characterization of Right Ventricular Myocardial Infarction Induced by Permanent Ligation of the Right Coronary Artery in Mice (Video) | JoVE Journal
Uploaded: 2022-02-01T14:50:00.000+00:00
Duration: 10 min 18 s
Description: Learn a reproducible method to generate right ventricular infarction in mice by ligating the right coronary artery. This technique enables researchers to study RVI pathophysiology and mechanisms in vivo.

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This work was supported by grants from the National Natural Science Foundation of China (82073851 to Sun) and the National China Postdoctoral Science Foundation (2021M690074 to Lin).

All procedures were performed according to the Guide for the Care and Use of Laboratory Animals published by the US National Institutes of Health (NIH Publication No. 85-23, revised in 1996) and were approved by the Animal Ethics Committee of Nanfang Hospital, Southern Medical University (Guangzhou, China). Healthy male C57BL/6J mice (8-10 weeks old; body weight, 25-30 g) were obtained from the Animal Center of Southern Medical University. Female mice can also be used, but mixing both genders is not recommended due to the potential influences of sex differences. After arrival, the mice were housed under a 12-h/12-h dark/light cycle (3-4 mice per cage), with ad libitum food and water.
1. Preparation for surgery
<ol>
	<li>Sterilize surgical instruments by autoclaving&#160;before the surgery. Adjust the heating pad to 37 &#176;C.</li>
	<li>Anesthetize the mice by an intraperitoneal injection of 50 mg/kg of pentobarbital (see Table of Materials) to relieve surgical pain. Place the mice in separate boxes for anesthesia induction. Ensure the depth of anesthesia by the absence of a toe-withdrawal response. 
	NOTE: It is also recommended to use 1.5% isoflurane for inhalation anesthesia because it is better for analgesia.</li>
	<li>Place the mice supine on the pad by fixing their incisors with a suture and immobilizing their limbs with adhesive tape. Ensure the depth of anesthesia again by checking the reflex.</li>
	<li>Remove hair from the neck to xiphoid with a depilatory cream. Disinfect the surgical area 3 times with alternating antiseptic scrub and 75% alcohol and&#160;then drape the surgical field.</li>
	<li>Perform intubation following the steps below.
	<ol>
		<li>Adjust the breathing frequency of the animal with a mini ventilator (see Table of Materials) to 150/min and the tidal volume to 300 &#181;L. 
		&#8203;NOTE: It is unnecessary to use positive end-expiratory pressure mode.</li>
		<li>Pull out the tongue slightly with tweezers, lift the mandible with a tongue depressor to expose the glottis, and perform intra-tracheal intubation by inserting a 22 G cannula into the glottis.</li>
		<li>Switch on the mini ventilator and connect the tracheal cannula to the ventilator. The phenomenon of thoracic undulation becoming equal to the ventilator frequency indicates successful intubation. Fix the cannula with tape to prevent slipping during the operation.</li>
	</ol>
	</li>
</ol>
2. Permanent ligation of the right coronary artery
<ol>
	<li>Connect the electrocardiography (ECG) electrodes (see Table of Materials) to the mouse limbs correctly and record the ECG. 
	NOTE: One of the II, III, or AVF lead is selected as a monitoring lead; Lead III is more appropriate.</li>
	<li>Open the chest.
	<ol>
		<li>Make a 1 cm long incision in the skin parallel to the third right rib with ophthalmic scissors. Determine the third intercostal again and ensure adequate space according to the sternum angle. 
		NOTE: The direction of the skin incision is made from the sternum angle to the right anterior axillary line.</li>
		<li>Separate and cut the pectoralis major and pectoralis minor muscles with scissors and micro forceps above the third intercostal space. After that, bluntly separate the intercostal muscle with elbow forceps to expose the surgical field. 
		NOTE: Only a small part of pectoral muscles needs to be cut, and then a blunt separation is recommended to expose the heart.</li>
		<li>Incise the pericardium. Lift the right atrium with sterile cotton and ligate the RCA with a sterile 8-0&#160;nylon thread with a ligation range of 3-5 mm. After ligating the RCA, the monitoring ECG (lead III) shows ST-segment elevation.&#160; 
		NOTE: Because the mouse RCA is invisible, its anatomic location must be carefully confirmed. The myocardium of the RV is much thinner than that of the LV. Therefore, it is difficult to grasp the depth of the inserted needle. It is easy to induce sinus bradycardia and atrioventricular block if the depth of the inserted needle is too deep and the ligation range is too large.</li>
	</ol>
	</li>
	<li>Remove the sterile cotton and&#160;suture muscles and skin with a sterile 5-0 nylon thread to close the intercostal incision.&#160;Disinfect the skin again with 75% alcohol and&#160;single-house the mouse&#160;after surgery. 
	NOTE: The well-sutured muscle is important for avoiding aerothorax. A sterile drainage tube is placed in the chest cavity until the completion of chest closing, and then the chest cavity is evacuated by an injection syringe connecting the drainage tube. 
	NOTE: After surgery, mice are placed on a heating&#160;pad. Analgesics such as buprenorphine (0.1 mg/kg body weight, subcutaneously injection) are required to reduce the animals&#39; pain after the surgery. The expected complications are sinus bradycardia and atrioventricular block, and the mortality rate post-surgery is 10-20%.</li>
</ol>
3. Echocardiographic assessment of the RV function after surgery
NOTE: For echocardiography, use an MS400D probe with a center frequency of 30 MHz, connected to a high-resolution ultrasound imaging system (see Table of Materials). The echocardiography examination is performed 4 weeks after surgery.
<ol>
	<li>Anesthetize the mouse with 3% isoflurane via inhalation.</li>
	<li>Place the mouse in the supine position on an ultrasonic platform for animal fixation and ultrasonic operation. Tape its claws to the electrode to obtain an ECG recording through a system attached to the ultrasonic machine.</li>
	<li>Monitor heart rate through ECG and maintain it between 450-550 beats/min by adjusting the anesthetic concentration between 1.5% and 3%.</li>
	<li>Remove the hair from the mouse&#39;s chest with a depilatory cream and apply ultrasound gel to the skin of the chest.</li>
	<li>Set the platform to the horizontal position. Orient the transducer parallel to the left leg and obtain the left ventricular long-axis image. Rotate the probe 90&#176; clockwise to obtain the LV short-axis view. Press the Cine store button to save the images. 
	NOTE: The upper-left of the platform is tilted at the lowest point. The LV short-axis rotation angle of the transducer is maintained while the transducer is oriented toward the right shoulder of the mouse.</li>
	<li>Move down the transducer vertically, maintaining its position over the upper abdomen and below the mouse&#39;s diaphragm under B-mode. Adjust the platform position slightly by rotating its x- and y-axes until the RV, right atrium (RA), left atrium (LA), and LV are clearly seen on the screen. Save apical four-chamber images by pressing the Cine store or Frame store button. 
	NOTE: B-mode is used to show the two-dimension (2D) view of the heart.</li>
	<li>Press M-mode; after the 2x indicator line appears, locate the indicator line at the tricuspid valve orifice to obtain the movement of the tricuspid annular plane. Press the Cine store or Frame store button to save data and images. 
	NOTE: M-mode means motion mode, which reveals the motion of the heart or vessel in a curve form.</li>
	<li>Press Measure button to enter measurement mode. Click on Area measurement button to zone into RV and LV. Calculate the area of RV and LV to obtain the area ratio of RV to LV.
	<ol>
		<li>Click on Timeline button and make two baselines to define the movement range of the tricuspid annular plane during the systolic and diastolic periods. Click on Distance button and measure the distance between two baselines to obtain tricuspid annular plane systolic excursion (TAPSE).</li>
	</ol>
	</li>
	<li>Tilt the left side of the platform at the lowest point. Keep the probe at a 30&#176; angle to the horizontal axis along the right anterior axillary line. Rotate the x- and y-axes of the platform to display the RV.
	<ol>
		<li>Press M-mode button and locate the indicator line at the septum&#39;s hyperechoic point to obtain the M-mode image of the RV interface. Press Cine store button to save the picture.</li>
	</ol>
	</li>
	<li>Open the M-mode image of the RV interface, press Measure button to enter measurement mode. Measure the RV inner distance at the end of diastole (RVIDd), RV ejection fraction (RVEF), and RV fraction shortening (RVFS) using the in-built measurement tool of the echocardiographic system.</li>
	<li>Stop administering isoflurane and place the mouse on the heating pad for 3-5 min until it regains consciousness. After that, return the mouse to its cage with 12 h light/dark cycle.</li>
</ol>
4. Invasive measurements of RV hemodynamic
NOTE: RV hemodynamic is assessed through right heart catheterization 4 weeks after RVI. A 1.0 F catheter together with a monitoring system is applied.
<ol>
	<li>Anesthetize the mouse with an intraperitoneal injection of 50 mg/kg of sodium pentobarbital (see Table of Materials).</li>
	<li>After confirming the disappearance of the pedal withdrawal reflex, keep the mouse in the supine position and immobilize it with adhesive tape.</li>
	<li>Shave the chest hair from the sternal angle to the xiphoid. Disinfect the operating area with 75% alcohol.</li>
	<li>Perform tracheal intubation and set the parameter of the animal ventilator as described in steps 1.5.2-1.5.3.</li>
	<li>Make a 1 cm bilateral incision on the skin above the xiphoid process and transect the diaphragm and rib with ophthalmic scissors to expose the heart.</li>
	<li>Puncture the right ventricular free wall with a 32 G needle. Remove the needle and press the wound with cotton to stanch bleeding.</li>
	<li>Insert the tip of the catheter into the right ventricle through the puncture site and push the catheter forward slowly. Adjust the position of the tip to obtain a typical RV pressure waveform shown on a monitor and recording system. 
	NOTE: Right jugular vein is also an appropriate route for hemodynamic measurement.</li>
	<li>After 10 min of stabilization, record the data of RV systolic blood pressure (RVSBP), RV end-diastolic pressure (RVEDP), and RV dP/dt. Click on Select button to select cardiac cycles for calculation and then click on Analyze button to calculate the mean values of the selected cycles.</li>
	<li>Remove the catheter after completion of recording and then place it inside normal saline solution.</li>
	<li>Euthanize the mouse with an intraperitoneal injection of overdose pentobarbital sodium (150 mg/kg) and then sacrifice it by cervical dislocation.</li>
	<li>Collect the heart and tibia for histological analysis.</li>
</ol>
5. Coronary vascular cast using a vascular casting agent
<ol>
	<li>Heparinize the mouse with an intraperitoneal injection of 200 IU/mL of heparin sodium at 2000 IU/kg (see Table of Materials).</li>
	<li>Anesthetize the mouse with an intraperitoneal injection of 50 mg/kg of sodium pentobarbital.</li>
	<li>Place the animal supine on the pad and intubate for artificial ventilation following steps 1.5.2-1.5.3.</li>
	<li>Open the chest with surgical scissors as described in step 4.5 and expose the heart.</li>
	<li>Make a 3 mm notch with ophthalmic scissors on the right atria and perfuse the heart with 5 mL of normal saline through the cardiac apex with an injector.</li>
	<li>Block the blood from the aorta with an aortic clamp and perfuse 0.1 mL of nitroglycerin (1 mg/mL) through the cardiac apex with an injector to dilate the coronary artery.</li>
	<li>Prepare the cast reagent by mixing the ingredients in the kit according to the manufacturer&#39;s instructions (see Table of Materials). 
	NOTE: It is recommended to prepare the cast reagent and perfusion with normal saline and nitroglycerin simultaneously to prevent microvascular closure.</li>
	<li>Perfuse the heart with 1 mL of cast reagent through the cardiac apex and wait for 2-3 h.</li>
	<li>Erode the heart with 50% sodium hydroxide for 2-3 days and remove the muscle tissue or connective tissue by rinsing with normal saline.</li>
	<li>Take pictures under a camera. 
	CAUTION: The cast reagent is harmful to the eyes, skin, and respiratory tract. Sodium hydroxide is corrosive. Wearing protective gloves, goggles, and a lab coat is required. The cast reagent must be prepared in a fume hood.</li>
</ol>

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</ol>

The authors have nothing to disclose.

Sicard and colleagues from France first reported a mouse model of RVI in 2019, which described the surgical process and focused on the interaction between LV and RV after RVI9. However, to date, no study has reported using this model for further studies. A more detailed procedure would be helpful for researchers to use the mouse model of RVI for investigation. In contrast to the report by Sicard et al.9, we provided step-by-step information for model generation and strategy...

The right ventricle (RV), long thought to be a simple tube connected to the pulmonary artery, has been wrongfully neglected for many years1. However, there has been an increasing interest in RV function recently since it plays an essential role in hemodynamic disorders2,3 and may serve as an independent risk predictor of cardiovascular disease4,5,6,7. RV diseases include RV infarction (RVI), pulmonary artery hypertension, and valvular disease8. In contrast to the immense interest in pulmonary artery hypertension, RVI has remained neglected7,9.
RVI, usually accompanied by inferior-posterior myocardial infarction10,11, is caused by right coronary artery (RCA) occlusion. According to clinical investigations, severe RVI likely induces hemodynamic disturbances and arrhythmias, such as hypotension, bradycardia, and atrioventricular block, associated with higher hospital morbidity and mortality12,13,14. RV function could recover spontaneously to a certain extent even in the absence of reperfusion15,16. Several morphological and functional differences exist between the left ventricle (LV) and RV17. RV is believed to be more resistant to ischemia than LV8, partially due to the more extensive collateral circulation formation after RVI. Clarifying the differences between LV infarction (LVI) and RVI and identifying the underlying mechanisms would provide new therapeutic targets for cardiac regeneration and ischemic heart failure. However, owing to the difficulty associated with&#160;RVI mouse model generation, basic research on RVI is mainly limited.
A large animal model of RVI has been generated by ligating RCA in swine18, which is easier to operate because of the visible RCA. Compared with the large animal model, the mouse model has the following advantages: more accessibility in gene manipulation, lower economic cost, and shorter experimental period19,20. Although a mouse RVI model focusing on the influence of RVI on LV function was reported previously, the detailed steps of the procedure, the difficulties and key points of operation, and the model characteristics such as hemodynamic changes were not fully introduced9,21.
This article provides detailed surgical procedures for generating a mouse model of RVI. Moreover, this model was characterized by echocardiographic measurement, invasive hemodynamic evaluation, and histological analysis. Furthermore, a coronary vasculature cast was performed to observe the coronary arterial arrangement in RV. The technique introduced in this paper would help beginners to quickly grasp the generation of the mouse RVI model with acceptable operation mortality and reliable evaluation approaches. The mouse model of RVI would help research the mechanisms of right heart failure and seek new therapeutic targets of RV remodeling.

<table><tbody><tr><td>2,3,5-triphenyltetrazolium chloride</td><td>Sigma</td><td>T8877</td><td>For TTC staining</td></tr><tr><td>Animal Mini Ventilator</td><td>Havard</td><td>Type 845</td><td>For artificial ventilation</td></tr><tr><td>Animal ultrasound system VEVO2100</td><td>Visual Sonic</td><td>VEVO2100</td><td>Measurement for Doppler flow velocity and AS plaque</td></tr><tr><td>Batson&amp;rsquo;s #17 Anatomical Corrosion Kit</td><td>Polyscience Inc</td><td>7349</td><td>For vasculature casting</td></tr><tr><td>buprenorphine</td><td>Isoreag</td><td>1134630-70-8</td><td>For reduce the pain of mice after surgery</td></tr><tr><td>C57BL/6J mice + D29A1A2:D27</td><td>Animal Center of South Medical University</td><td>-</td><td>For the generation of mouse RVI model</td></tr><tr><td>Camera</td><td>Sangnond</td><td></td><td>For taking photograph</td></tr><tr><td>Cold light illuminator</td><td>Olympus</td><td>ILD-2</td><td>Light for operation</td></tr><tr><td>electrocardiograph</td><td>ADI Instrument</td><td>ADAS1000</td><td>For recording electrocardiogram</td></tr><tr><td>hair removal cream</td><td>Reckitt Benchiser</td><td>RQ/B 33 Type 2</td><td>Remove mouse hair</td></tr><tr><td>Heat pad- thermostatic surgical system (ALC-HTP-S1)</td><td>SHANGHAI ALCOTT BIOTECH CO</td><td>ALC-HTP-S1</td><td>Heating</td></tr><tr><td>Hematoxylin-eosin dye</td><td>Leagene</td><td>DH0003</td><td>Hematoxylin-eosin staining</td></tr><tr><td>Heparin sodium salt</td><td>Macklin</td><td>H837056</td><td>For heparization</td></tr><tr><td>Isoflurane</td><td>RWD life science</td><td>R510-22</td><td>Inhalant anaesthesia</td></tr><tr><td>Lab made spatula</td><td></td><td></td><td>Work as a laryngoscope</td></tr><tr><td>Lab made tracheal cannula</td><td></td><td></td><td>For intubation</td></tr><tr><td>Matrx VIP 3000 Isofurane Vaporizer</td><td>Midmark Corporation</td><td>VIP 3000</td><td>Anesthetization</td></tr><tr><td>Medical nylon suture (5-0)</td><td>Ningbo Medical Needle Co.</td><td>5-0</td><td>For chest close</td></tr><tr><td>Microsurgical elbow tweezers</td><td>RWD life science</td><td>F11021-11</td><td>For surgery</td></tr><tr><td>Microsurgical scissors</td><td>NAPOX</td><td>MB-54-1</td><td>For arteriotomy</td></tr><tr><td>Millar Catheter</td><td>AD Instruments, Shanghai</td><td>1.0F</td><td>Measurement of pressure gradient</td></tr><tr><td>MS400D ultrasonic probe</td><td>Visual Sonic</td><td>MS400D</td><td>Measurement for Doppler flow velocity and AS plaque</td></tr><tr><td>needle forceps</td><td>Visual Sonic</td><td>F31006-12</td><td>For surgery</td></tr><tr><td>nitroglycerin</td><td>BEIJING YIMIN MEDICINE Co</td><td></td><td>For dilating coronary artery</td></tr><tr><td>Ophthalmic scissors</td><td>RWD life science</td><td>S11022-14</td><td>For surgery</td></tr><tr><td>Pentobarbital sodium salt</td><td>Merck</td><td>25MG</td><td>Anesthetization</td></tr><tr><td>PowerLab Multi-Directional Physiological Recording System</td><td>AD Instruments, Shanghai</td><td>4/35</td><td>Pressure recording</td></tr><tr><td>Precision electronic balance</td><td>Denver Instrument</td><td>TB-114</td><td>Weighing scale</td></tr><tr><td>Silk suture (8-0)</td><td>Ningbo Medical Needle Co.</td><td>6-0</td><td>coronary artery ligation</td></tr><tr><td>Small animal microsurgery equipment</td><td>Napox</td><td>MA-65</td><td>Surgical instruments</td></tr><tr><td>tissue forceps</td><td>Visual Sonic</td><td>F-12007-10</td><td>For surgery</td></tr><tr><td>tissue scissor</td><td>Visual Sonic</td><td>S13052-12</td><td>Open chest for hemodynamic measurement</td></tr><tr><td>Transmission Gel</td><td>Guang Gong pai</td><td>250ML</td><td>preparation for Echocardiography measurement</td></tr><tr><td>Vascular Clamps</td><td>Visual Sonic</td><td>R31005-06</td><td>For blocking blood from aorta</td></tr></tbody></table>

generation and characterization of right ventricular myocardial infarction induced by permanent ligation of the right coronary artery in mice

Generation and Characterization of Right Ventricular Myocardial Infarction Induced by Permanent Ligation of the Right Coronary Artery in Mice

Right ventricular infarction (RVI) is a common presentation in clinical practice. Severe RVI can lead to fatal hemodynamic dysfunction and arrhythmia. In contrast to the extensively used mouse myocardial infarction (MI) model generated by left coronary artery ligation, the RVI mouse model is rarely employed due to the difficulty associated with model generation. Research on the mechanisms and treatment of RVI-induced RV remodeling and dysfunction requires animal models to mimic the pathophysiology of RVI in patients. This study introduces a feasible procedure for RVI model generation in C57BL/6J mice. Further, this model was characterized based on the following: infarct size evaluation at 24 h after MI, assessment of cardiac remodeling and function with echocardiography, RV hemodynamics assessment, and histology of the infarct zone at 4 weeks after RVI. In addition, a coronary vasculature cast was performed to observe the coronary arterial arrangement in RV. This mouse model of RVI would facilitate the research on mechanisms of right heart failure and seek new therapeutic targets of RV remodeling.

In this study, mice were randomly assigned to the RVI (n = 11) or sham operation (n = 11) group. The coronary cast in 2 normal mouse hearts is shown in Figure 1A. In response to RCA ligation, ST-segment elevation was seen in lead III of the ECG (Figure 1B). Moreover, 2,3,5-triphenyl tetrazolium chloride (TTC) staining showed that the infarct area accounts for 45% of the RV free wall at 24 h postoperatively (Figure 1C,D</stro...

There are several differences between the right and left ventricles. However, the pathophysiology of right ventricular infarction (RVI) has not been clarified. In the present protocol, a reproducible method for RVI mouse model generation is introduced, which may provide a means to explain the mechanism of RVI.

Right ventricular infarction (RVI) is a common presentation in clinical practice. Severe RVI can lead to fatal hemodynamic dysfunction and arrhythmia. In ...

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JoVE Journal

Medicine

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Generation and Characterization of Right Ventricular Myocardial Infarction Induced by Permanent Ligation of the Right Coronary Artery in Mice (Video) | JoVE Journal

Generation and Characterization of Right Ventricular Myocardial Infarction Induced by Permanent Ligation of the Right Coronary Artery in Mice

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