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1Marsico Lung Institute / CF Center,The University of North Carolina at Chapel Hill, 2Rheonova, 3Department of Pulmonary and Critical Care Medicine,The University of North Carolina at Chapel Hill, 4Department of Pediatric, Pediatric Pulmonology Division,The University of North Carolina at Chapel Hill
Chapters
- 00:04Introduction
- 01:22Sputum or Mucus Collection and Storage
- 02:11Sample Preparation
- 03:17Instrument Initialization and Calibration
- 04:21Sample Loading
- 05:49Initiate Biophysical Measurement and Sample Removal
- 06:33Results: Using Benchtop Rheometer to Monitor Changes in Mucus Viscoelasticity
- 08:05Conclusion
The viscoelastic properties of mucus play a critical role in mucociliary clearance. However, traditional mucus rheological techniques require complex and time-consuming approaches. This study provides a detailed protocol for the use of a benchtop rheometer that can rapidly and reliably perform viscoelastic measurements.
