Journal
/
Bioengineering
/
High Throughput Traction Force Microscopy Using PDMS Reveals Dose-Dependent Effects of Transforming Growth Factor-β on the Epithelial-to-Mesenchymal Transition
JoVE Journal
Bioengineering
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
High Throughput Traction Force Microscopy Using PDMS Reveals Dose-Dependent Effects of Transforming Growth Factor-β on the Epithelial-to-Mesenchymal Transition

High Throughput Traction Force Microscopy Using PDMS Reveals Dose-Dependent Effects of Transforming Growth Factor-β on the Epithelial-to-Mesenchymal Transition

DOI:
10.3791/59364-v

•

13:34 min

•

June 01, 2019

•

, , , , ,
1Department of Bioengineering,McGill University, 2Goodman Cancer Research Centre,McGill University, 3Department of Medicine,McGill University, 4Department of Emergency Medicine,Beth Israel Deaconess Medical Center

Chapters

  • 00:00Title
  • 02:45PDMS Rheometry
  • 10:42Surface Functionalization
  • 11:21Plating Cells
  • 11:48Data Acquisition
  • 12:19Cell Removal
  • 13:02Conclusion

Summary

Automatic Translation

Automatic Translation

We present a high throughput traction force assay fabricated with silicone rubber (PDMS). This novel assay is suitable for studying physical changes in cell contractility during various biological and biomedical processes and diseases. We demonstrate this method's utility by measuring a TGF-β dependent increase in contractility during the epithelial-to-mesenchymal transition.

Tags

Keywords: Traction Force MicroscopyHigh ThroughputPDMSEpithelial-to-mesenchymal TransitionTransforming Growth Factor-βCell ContractilityCancer MetastasisMechanobiologyRheometerSoft Substrate

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/59364?article_type=v

Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy
check_url/59364?article_type=v

High-throughput Protein Expression Generator Using a Microfluidic Platform
check_url/59364?article_type=v

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery
check_url/59364?article_type=v

Measuring the Mechanical Properties of Living Cells Using Atomic Force Microscopy
check_url/59364?article_type=v

Stretching Micropatterned Cells on a PDMS Membrane
check_url/59364?article_type=v

A Novel Method for Localizing Reporter Fluorescent Beads Near the Cell Culture Surface for Traction Force Microscopy
check_url/59364?article_type=v

Surface Potential Measurement of Bacteria Using Kelvin Probe Force Microscopy
check_url/59364?article_type=v

A High-throughput Cell Microarray Platform for Correlative Analysis of Cell Differentiation and Traction Forces
check_url/59364?article_type=v

Fabrication and Implementation of a Reference-Free Traction Force Microscopy Platform
check_url/59364?article_type=v

Traction Force Microscopy to Study B Lymphocyte Activation

Read Article