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High Throughput Traction Force Microscopy Using PDMS Reveals Dose-Dependent Effects of Transforming Growth Factor-β on the Epithelial-to-Mesenchymal Transition

Article DOI: 10.3791/59364-v • 13:34 min • June 1st, 2019
June 1st, 2019 •
1, 1, 1, 2,3, 4, 1,2
1Department of Bioengineering, McGill University, 2Goodman Cancer Research Centre, McGill University, 3Department of Medicine, McGill University, 4Department of Emergency Medicine, Beth Israel Deaconess Medical Center

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Summary
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We present a high throughput traction force assay fabricated with silicone rubber (PDMS). This novel assay is suitable for studying physical changes in cell contractility during various biological and biomedical processes and diseases. We demonstrate this method's utility by measuring a TGF-β dependent increase in contractility during the epithelial-to-mesenchymal transition.

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High Throughput Traction Force Microscopy PDMS Dose-dependent Effects Transforming Growth Factor-beta Epithelial-to-mesenchymal Transition Mechanobiology Cell Contractility Traction Force Microscopy (TFM) Multiwell Dishes Increased Throughput Time Efficiency Reagent Efficiency 96 Well Traction Force Dish Cancer Metastasis
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