Journal
/
Biology
/
Characterizing Epithelial Wound Healing In Vivo Using the Cnidarian Model Organism Clytia hemisphaerica
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Characterizing Epithelial Wound Healing In Vivo Using the Cnidarian Model Organism Clytia hemisphaerica

Characterizing Epithelial Wound Healing In Vivo Using the Cnidarian Model Organism Clytia hemisphaerica

DOI:
10.3791/65081-v

•

07:47 min

•

February 10, 2023

•

, ,
1Department of Molecular Genetics and Cell Biology,The University of Chicago

Chapters

  • 00:05Introduction
  • 00:53Procedure for Wounding: Microwounds and Small Wounds
  • 02:13Procedure for Wounding: Large Wounds
  • 03:24Imaging Epithelial Wound Healing
  • 03:52Procedure for Mesogleal Injections
  • 05:32Results: The Epithelial Wound Healing Process
  • 07:08Conclusion

Summary

Automatic Translation

Automatic Translation

This paper describes a method to create wounds in the epithelium of a live Clytia hemisphaerica medusa and image wound healing at a high resolution in vivo. Additionally, a technique to introduce dyes and drugs to perturb signaling processes in the epithelial cells and extracellular matrix during wound healing is presented.

Tags

Keywords: Epithelial Wound HealingClytia HemisphaericaIn VivoLive ImagingMicro-woundsScratch WoundsMicroinjectionCell MigrationCell SpreadingExtracellular MatrixCell Cytoplasm

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/65081?article_type=v

Vibrio cholerae: Model Organism to Study Bacterial Pathogenesis – Interview
check_url/65081?article_type=v

Microinjection of Medaka Embryos for use as a Model Genetic Organism
check_url/65081?article_type=v

Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation
check_url/65081?article_type=v

Chromatin Isolation by RNA Purification (ChIRP)
check_url/65081?article_type=v

Rapid Genetic Analysis of Epithelial-Mesenchymal Signaling During Hair Regeneration
check_url/65081?article_type=v

Culturing of Human Nasal Epithelial Cells at the Air Liquid Interface
check_url/65081?article_type=v

Using Caenorhabditis elegans as a Model System to Study Protein Homeostasis in a Multicellular Organism
check_url/65081?article_type=v

Visualizing Cytoplasmic Flow During Single-cell Wound Healing in Stentor coeruleus
check_url/65081?article_type=v

Investigating the Spreading and Toxicity of Prion-like Proteins Using the Metazoan Model Organism C. elegans
check_url/65081?article_type=v

In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability

Read Article