Placement of Extracranial Stimulating Electrodes and Measurement of Cerebral Blood Flow and Intracranial Electrical Fields in Anesthetized Mice

Simone Degan; Yu Feng; Ulrike Hoffmann; Dennis  A. Turner

doi:10.3791/65195

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Placement of Extracranial Stimulating Electrodes and Measurement of Cerebral Blood Flow and Intracranial Electrical Fields in Anesthetized Mice

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Placement of Extracranial Stimulating Electrodes and Measurement of Cerebral Blood Flow and Intracranial Electrical Fields in Anesthetized Mice

1,090 Views

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06:34 min

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June 02, 2023

DOI:

10.3791/65195-v

DOI:

10.3791/65195-v

•

06:34 min

June 02, 2023

•

1077 Views

Simone Degan¹, Yu Feng¹, Ulrike Hoffmann², Dennis A. Turner^1,3,4,5

¹Department of Neurosurgery,Duke University Medical Center ²Department of Anesthesiology and Pain Management,University Texas Southwestern Medical School ³Department of Neurobiology,Duke University Medical Center ⁴Department of Biomedical Engineering,Duke University ⁵Research and Surgery Services,Durham VA Medical Center

Summary

We describe a protocol for assessing dose-response curves for extracranial stimulation in terms of brain electrical field measurements and a relevant biomarker-cerebral blood flow. Since this protocol involves invasive electrode placement into the brain, general anesthesia is needed, with spontaneous breathing preferred rather than controlled respirations.