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Journal
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Biology
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Improved Protein Quantification Using Two-Step Mitochondrial Isolation
/
线粒体的两步无标签分离，用于改进蛋白质发现和定量
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Two-Step Tag-Free Isolation of Mitochondria for Improved Protein Discovery and Quantification
Please note that all translations are automatically generated.
Click here for the English version.
线粒体的两步无标签分离，用于改进蛋白质发现和定量
Improved Protein Quantification Using Two-Step Mitochondrial Isolation
DOI:
10.3791/65252-v
•
01:45 min
•
June 02, 2023
•
Joan Blanco-Fernandez
,
Alexis A. Jourdain
1
Department of Immunobiology
,
University of Lausanne
Tags
Keywords: Mitochondrial Isolation
Two-step Purification
Differential Centrifugation
Immune Capture
Mass Spectrometry
Proteomics
Metabolomics
CRISPR-Cas9
Mitochondrial Function
Energy Metabolism
Immune Response
Cancer
Metabolic Disorders
Anti-inflammatory Effects
Mitochondrial Proteome
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