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Journal
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Biology
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Improved Protein Quantification Using Two-Step Mitochondrial Isolation
/
Aislamiento de dos pasos sin etiquetas de las mitocondrias para mejorar el descubrimiento y la cuantificación de proteínas
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Two-Step Tag-Free Isolation of Mitochondria for Improved Protein Discovery and Quantification
Please note that all translations are automatically generated.
Click here for the English version.
Aislamiento de dos pasos sin etiquetas de las mitocondrias para mejorar el descubrimiento y la cuantificación de proteínas
Improved Protein Quantification Using Two-Step Mitochondrial Isolation
DOI:
10.3791/65252-v
•
01:45 min
•
June 02, 2023
•
Joan Blanco-Fernandez
,
Alexis A. Jourdain
1
Department of Immunobiology
,
University of Lausanne
Tags
Keywords: Mitochondrial Isolation
Two-step Purification
Differential Centrifugation
Immune Capture
Mass Spectrometry
Proteomics
Metabolomics
CRISPR-Cas9
Mitochondrial Function
Energy Metabolism
Immune Response
Cancer
Metabolic Disorders
Anti-inflammatory Effects
Mitochondrial Proteome
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