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Intracellular Signaling Events and Hormone Secretion in Human Pseudoislets

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Système de pseudo-îlots humains pour l’évaluation synchrone de la dynamique des biocapteurs fluorescents et des profils de sécrétion hormonale

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Biology

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JoVE Journal Biology

Human Pseudoislet System for Synchronous Assessment of Fluorescent Biosensor Dynamics and Hormone Secretory Profiles

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Système de pseudo-îlots humains pour l’évaluation synchrone de la dynamique des biocapteurs fluorescents et des profils de sécrétion hormonale

Intracellular Signaling Events and Hormone Secretion in Human Pseudoislets

DOI:

10.3791/65259-v

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01:38 min

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November 03, 2023

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Tiffany M. Richardson*¹, Yasminye D. Pettway*¹, John T. Walker, Heather A. Nelson, Matthew Ishahak, Gregory Poffenberger, Radhika Aramandla, Conrad Reihsmann, Ashutosh Agarwal, Alvin C. Powers^2,4, Marcela Brissova

¹Department of Molecular Physiology and Biophysics,Vanderbilt University School of Medicine, ²Division of Diabetes, Endocrinology and Metabolism, Department of Medicine,Vanderbilt University Medical Center, ³Department of Biomedical Engineering,University of Miami, ⁴VA Tennessee Valley Healthcare System

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Keywords: Human Pseudoislet Biosensor Dynamics Hormone Secretory Profiles Type 1 Diabetes Type 2 Diabetes Pancreatic Islet Beta Cell Dysfunction 3D Cell Culture Live-cell Imaging Microfluidic Platform Intracellular Signaling CAMP Insulin Secretion Glucagon Secretion Glucose Homeostasis

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Système de pseudo-îlots humains pour l’évaluation synchrone de la dynamique des biocapteurs fluorescents et des profils de sécrétion hormonale

Synchronous Assessment of Fluorescent Biosensor Dynamics and Hormone Profiles in Human Pseudoislets

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