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Intracellular Signaling Events and Hormone Secretion in Human Pseudoislets

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Humant pseudoisletsystem for synkron vurdering av fluorescerende biosensordynamikk og hormonsekretoriske profiler

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Biology

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JoVE Journal Biology

Human Pseudoislet System for Synchronous Assessment of Fluorescent Biosensor Dynamics and Hormone Secretory Profiles

Humant pseudoisletsystem for synkron vurdering av fluorescerende biosensordynamikk og hormonsekretoriske profiler

Intracellular Signaling Events and Hormone Secretion in Human Pseudoislets

DOI:

10.3791/65259-v

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01:38 min

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November 03, 2023

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Tiffany M. Richardson*¹, Yasminye D. Pettway*¹, John T. Walker, Heather A. Nelson, Matthew Ishahak, Gregory Poffenberger, Radhika Aramandla, Conrad Reihsmann, Ashutosh Agarwal, Alvin C. Powers^2,4, Marcela Brissova

¹Department of Molecular Physiology and Biophysics,Vanderbilt University School of Medicine, ²Division of Diabetes, Endocrinology and Metabolism, Department of Medicine,Vanderbilt University Medical Center, ³Department of Biomedical Engineering,University of Miami, ⁴VA Tennessee Valley Healthcare System

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Keywords: Human Pseudoislet Biosensor Dynamics Hormone Secretory Profiles Type 1 Diabetes Type 2 Diabetes Pancreatic Islet Beta Cell Dysfunction 3D Cell Culture Live-cell Imaging Microfluidic Platform Intracellular Signaling CAMP Insulin Secretion Glucagon Secretion Glucose Homeostasis

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Humant pseudoisletsystem for synkron vurdering av fluorescerende biosensordynamikk og hormonsekretoriske profiler

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