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Journal
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Biology
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Imaging of Mitochondrial Peroxide in Living Yeast Cells Using mtHyper7
/
जीवित खमीर कोशिकाओं में माइटोकॉन्ड्रियल पेरोक्साइड के लिए एक अनुपातमीट्रिक बायोसेंसर, एमटीहाइपर 7 की इमेजिंग
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Imaging of mtHyPer7, a Ratiometric Biosensor for Mitochondrial Peroxide, in Living Yeast Cells
Please note that all translations are automatically generated.
Click here for the English version.
जीवित खमीर कोशिकाओं में माइटोकॉन्ड्रियल पेरोक्साइड के लिए एक अनुपातमीट्रिक बायोसेंसर, एमटीहाइपर 7 की इमेजिंग
Imaging of Mitochondrial Peroxide in Living Yeast Cells Using mtHyper7
DOI:
10.3791/65428-v
•
02:28 min
•
June 02, 2023
•
Emily Jie-Ning Yang
,
Istvan R. Boldogh
2
,
Haojie Ji
,
Liza Pon
2
,
Theresa C. Swayne
2
1
Department of Pathology and Cell Biology
,
Columbia University Irving Medical Center
,
2
Confocal and Specialized Microscopy Shared Resource in the Herbert Irving Comprehensive Cancer Center
,
Columbia University Irving Medical Center
Tags
Keywords: Mitochondria
Biosensor
MtHyPer7
Hydrogen Peroxide
Reactive Oxygen Species
Live Cell Imaging
Yeast
Saccharomyces Cerevisiae
Mitochondrial Function
Mitochondrial Quality Control
Cell Fitness
Lifespan
Ratiometric
Genetically Encoded
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check_url/65428?article_type=v
जीवित खमीर कोशिकाओं में माइटोकॉन्ड्रियल पेरोक्साइड के लिए एक अनुपातमीट्रिक बायोसेंसर, एमटीहाइपर 7 की इमेजिंग
check_url/65428?article_type=v
इमेजिंग के लिए खमीर कोशिकाओं की तैयारी, इमेजिंग स्थितियों का अनुकूलन, और छवि संग्रह
check_url/65428?article_type=v
स्क्रिप्ट के साथ खमीर कोशिकाओं में mtHyPer7 का अर्ध-स्वचालित विश्लेषण
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