RGBradford: Protein Quantitation with a Smartphone Camera

Daniel C. Moreira

doi:10.3791/65547

Journal

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Biochemistry

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RGBradford: Protein Quantitation with a Smartphone Camera

JoVE Journal
Biochemistry

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JoVE Journal Biochemistry

RGBradford: Protein Quantitation with a Smartphone Camera

2,849 Views

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07:41 min

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September 08, 2023

DOI:

10.3791/65547-v

DOI:

10.3791/65547-v

•

07:41 min

September 08, 2023

•

70 Views

Daniel C. Moreira¹

¹Research Center in Morphology and Applied Immunology, Faculty of Medicine,University of Brasilia

Transcript

Our research focuses on understanding at the biochemical level how animals adapt to extreme environmental conditions. We explore questions about their response to lack of oxygen and suppression of metabolic rate. Specifically, we investigate endogenous antioxidants, oxidative stress, and cellularity stress response animals employ in a adverse environment.

And to do so, we often quantify protein levels in our samples. This protocol addresses the need for an alternative method to obtain protein quantification data when resource are limited, making it a valuable tool in both educational and research contexts. The RG Bradford Assay offers a practical and reliable solution for protein concentration measurement that is accessible, simple, and accurate in scenarios where conventional equipment such as a microplate reader may be unavailable.

Summary

This paper provides a protocol for protein quantification using the Bradford assay and a smartphone as an analytical device. Protein levels in samples can be quantified using color data extracted from a picture of a microplate taken with a smartphone.