Rapid <em>In Vitro</em> Cytotoxicity Evaluation of Jurkat Expressing Chimeric Antigen Receptor using Fluorescent Imaging

Siddharth Subham; John D. Jeppson; Benjamin K. Gibbs; Jacqueline Babai; Riza Alker; Andrew K. Godwin; David Akhavan

doi:10.3791/65560

Journal

/

Cancer Research

/

Rapid In Vitro Cytotoxicity Evaluation of Jurkat Expressing Chimeric Antigen Receptor using Fluorescent Imaging

Rapid <em>In Vitro</em> Cytotoxicity Evaluation of Jurkat Expressing Chimeric Antigen Receptor using Fluorescent Imaging

JoVE Journal
Cancer Research

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Cancer Research

Rapid In Vitro Cytotoxicity Evaluation of Jurkat Expressing Chimeric Antigen Receptor using Fluorescent Imaging

Rapid In Vitro Cytotoxicity Evaluation of Jurkat Expressing Chimeric Antigen Receptor using Fluorescent Imaging

2,215 Views

•

09:20 min

•

October 27, 2023

DOI:

10.3791/65560-v

DOI:

10.3791/65560-v

•

09:20 min

October 27, 2023

•

2203 Views

Siddharth Subham*^1,2,3, John D. Jeppson*¹, Benjamin K. Gibbs⁴, Jacqueline Babai², Riza Alker¹, Andrew K. Godwin^4,5,6, David Akhavan^1,2,3

¹Department of Radiation Oncology,University of Kansas Cancer Center ²Department of Cancer Biology,University of Kansas Cancer Center ³BioEngineering Program,University of Kansas ⁴Department of Pathology and Laboratory Medicine,University of Kansas Medical Center ⁵University of Kansas Cancer Center ⁶Kansas Institute for Precision Medicine,University of Kansas Medical Center

Summary

A protocol to evaluate quantitative tumor cell killing by Jurkat cells expressing chimeric antigen receptor (CAR) targeting single tumor antigen. This protocol can be used as a screening platform for rapid optimization of CAR hinge constructs prior to confirmation in peripheral blood-derived T cells.