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June 16, 2011
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Cell-mediated lymphocytotoxicity (CML) assays can be used to test autoreactive responses and study mechanisms of cell death in vitro. However, using live-cell confocal microscopic imaging techniques with fluorescent dyes, the type and kinetics of cell death as well as the pathways utilized can be studied in greater detail.
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Cite this Article
Chen, J., Grieshaber, S., Mathews, C. E. Methods to Assess Beta Cell Death Mediated by Cytotoxic T Lymphocytes. J. Vis. Exp. (52), e2724, doi:10.3791/2724 (2011).
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