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Methods to Assess Beta Cell Death Mediated by Cytotoxic T Lymphocytes
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Chapters
- 00:05Title
- 01:38Introduction
- 02:29Cell Culture
- 04:42Cell-mediated Lymphocytotoxicity (CML Assay)
- 07:29Live Cell Imaging to Asses CTL-mediated Beta Cell Death
- 10:10Representative Cell Death Assay Data
- 11:39Conclusion
Cell-mediated lymphocytotoxicity (CML) assays can be used to test autoreactive responses and study mechanisms of cell death in vitro. However, using live-cell confocal microscopic imaging techniques with fluorescent dyes, the type and kinetics of cell death as well as the pathways utilized can be studied in greater detail.
Tags
Beta Cell DeathCytotoxic T LymphocytesType 1 DiabetesInsulin ProductionMechanisms Of Beta Cell DeathCell-mediated Lymphocytotoxicity Assay51Cr ReleaseEffector CellsAutoreactive CD8+ Cytotoxic T LymphocytesAI4 T Cell ReceptorSpecific LysisMitochondriaMitochondrial Functional ChangesMitochondrial Membrane Potential Dye
