Quantitative Polymerase Chain Reaction (qPCR)-Based Rapid Diagnosis of <em>Helicobacter pylori</em> Infection and Antibiotic Resistance

Liang Wang; Jin-Xin Lai; Yu-Ting Si; Xu-Xia Cui; Zeeshan Umar; Xiao-Jun Ru; Xin-Yu Zhang; Zheng-Kang Li; Alfred Chin Yen Tay; Barry J. Marshall; Guang-Hua Li; Bing Gu

doi:10.3791/65689

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Quantitative Polymerase Chain Reaction (qPCR)-Based Rapid Diagnosis of Helicobacter pylori Infection and Antibiotic Resistance

Quantitative Polymerase Chain Reaction (qPCR)-Based Rapid Diagnosis of <em>Helicobacter pylori</em> Infection and Antibiotic Resistance

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Quantitative Polymerase Chain Reaction (qPCR)-Based Rapid Diagnosis of Helicobacter pylori Infection and Antibiotic Resistance

Quantitative Polymerase Chain Reaction (qPCR)-Based Rapid Diagnosis of Helicobacter pylori Infection and Antibiotic Resistance

3,214 Views

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06:40 min

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July 28, 2023

DOI:

10.3791/65689-v

DOI:

10.3791/65689-v

•

06:40 min

July 28, 2023

•

3163 Views

Liang Wang*^1,2,3, Jin-Xin Lai*¹, Yu-Ting Si*^1,4, Xu-Xia Cui^1,4, Zeeshan Umar^1,5, Xiao-Jun Ru¹, Xin-Yu Zhang¹, Zheng-Kang Li¹, Alfred Chin Yen Tay^5,6,7,8, Barry J. Marshall^5,6,7,8, Guang-Hua Li¹, Bing Gu¹

¹Laboratory Medicine, Guangdong Provincial People’s Hospital (Guangdong Academy of Medical Sciences),Southern Medical University ²Division of Microbiology and Immunology, School of Biomedical Sciences,The University of Western Australia ³Center for Precision Health, School of Medical and Health Sciences,Edith Cowan University ⁴Medical Technology School of Xuzhou Medical University ⁵Marshall Laboratory of Biomedical Engineering, School of Medicine,Shenzhen University ⁶The Marshall Centre for Infectious Diseases Research and Training,The University of Western Australia ⁷Marshall International Digestive Diseases Hospital,Zhengzhou University ⁸Marshall Medical Research Center,Fifth Affiliated Hospital of Zhengzhou University

Summary

The protocol presents a noninvasive method for the rapid diagnosis of Helicobacter pylori stomach infections through the string test and determines its antibiotic resistance to clarithromycin and levofloxacin using quantitative polymerase chain reaction (qPCR).