Targeted Metabolomics on Rare Primary Cells

Katharina M. Glaser; Mirijam Egg; Sebastian Hobitz; Michael Mitterer; Dominik Schain-Zota; Katharina Sch&#246;nberger; Konrad Schuldes; Nina Cabezas-Wallscheid; Tim L&#228;mmermann; Angelika Rambold; Joerg M. Buescher

doi:10.3791/65690

Journal

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Immunology and Infection

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Targeted Metabolomics on Rare Primary Cells

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Targeted Metabolomics on Rare Primary Cells

1,856 Views

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08:28 min

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February 23, 2024

DOI:

10.3791/65690-v

DOI:

10.3791/65690-v

•

08:28 min

February 23, 2024

•

1838 Views

Katharina M. Glaser*¹, Mirijam Egg*¹, Sebastian Hobitz¹, Michael Mitterer¹, Dominik Schain-Zota¹, Katharina Schönberger¹, Konrad Schuldes¹, Nina Cabezas-Wallscheid¹, Tim Lämmermann¹, Angelika Rambold¹, Joerg M. Buescher¹

¹Max Planck Institute of Immunobiology and Epigenetics

Summary

Here, we present a protocol to accurately and reliably measure metabolites in rare cell types. Technical improvements, including a modified sheath fluid for cell sorting and the generation of relevant blank samples, enable a comprehensive quantification of metabolites with an input of only 5000 cells per sample.