Disposable Dosators Intended for Dry Powder Delivery to Mice

Sara E. Maloney; Ian E. Stewart; Jeffrey B. Mecham; Mercedes Gonzalez-Juarrero; Bernd Meibohm; Anthony J. Hickey

doi:10.3791/65756

Journal

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Medicine

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Disposable Dosators Intended for Dry Powder Delivery to Mice

JoVE Journal
Medicine

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JoVE Journal Medicine

Disposable Dosators Intended for Dry Powder Delivery to Mice

756 Views

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04:59 min

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August 18, 2023

DOI:

10.3791/65756-v

DOI:

10.3791/65756-v

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04:59 min

August 18, 2023

•

1 Views

Sara E. Maloney¹, Ian E. Stewart¹, Jeffrey B. Mecham¹, Mercedes Gonzalez-Juarrero², Bernd Meibohm³, Anthony J. Hickey¹

¹Technology Advancement and Commercialization,RTI International, ²Mycobacteria Research Laboratories, Department of Microbiology, Immunology, and Pathology,Colorado State University, ³Department of Pharmaceutical Sciences, College of Pharmacy,University of Tennessee Health Science Center

Transcript

We’re developing new and existing drugs for inhalational therapy of mycobacterial disease. Our focus is on a novel approach to treat drug-resistant organisms, ensuring safety and efficacy using dry powders. The initial preclinical testing of dry powder inhaler products typically occurs in a murine model prior to movement to a larger animal model.

A significant limitation to this approach is the lack of suitable device technology for accurate and reproducible delivery of dry powders to small animals. The disposable dosators that we have developed are easy to manufacture, making them accessible and cost-effective for initial proof-of-concept studies in mice. The disposable nature allows their use in animal procedure rooms without concerns for cleaning, refilling, and weighing out materials for reusable devices.

Our future work will employ biotherapeutic moieties targeted to the pathogen that will avoid any effects on the host. This approach then can be tailored to the patient because it’s pathogen specific.

Summary

Pharmaceutical dry powder development necessitates reliable in vivo testing, often using a murine model. Device technology for accurately and reproducibly delivering dry powder aerosols to mice is restricted. This study presents disposable dosators for pulmonary drug delivery at mouse-relevant doses, aiding initial proof-of-concept research.