Using <em>Drosophila</em> Larval Neuromuscular Junction and Muscle Cells to Visualize Microtubule Network

Shengquan Zhang; Xiongxiong Wang; Zhihua Liu; Shan Jin; Chuan-Xi Mao

doi:10.3791/65774

Journal

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Neuroscience

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Using Drosophila Larval Neuromuscular Junction and Muscle Cells to Visualize Microtubule Network

Using <em>Drosophila</em> Larval Neuromuscular Junction and Muscle Cells to Visualize Microtubule Network

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Using Drosophila Larval Neuromuscular Junction and Muscle Cells to Visualize Microtubule Network

Using Drosophila Larval Neuromuscular Junction and Muscle Cells to Visualize Microtubule Network

2,650 Views

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08:04 min

•

October 20, 2023

DOI:

10.3791/65774-v

DOI:

10.3791/65774-v

•

08:04 min

October 20, 2023

•

2594 Views

Shengquan Zhang¹, Xiongxiong Wang¹, Zhihua Liu¹, Shan Jin¹, Chuan-Xi Mao¹

¹National & Local Joint Engineering Research Center of High-throughput Drug Screening Technology, School of life science,Hubei University

Summary

Here, we present a detailed protocol to visualize the microtubule networks in neuromuscular junctions and muscle cells. Combined with the powerful genetic tools of Drosophila melanogaster, this protocol greatly facilitates genetic screening and microtubule-dynamics analysis for the role of microtubule network regulatory proteins in the nervous system.