Using <em>Drosophila</em> Larval Neuromuscular Junction and Muscle Cells to Visualize Microtubule Network

Shengquan Zhang; Xiongxiong Wang; Zhihua Liu; Shan Jin; Chuan-Xi Mao

doi:10.3791/65774

Journal

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Neuroscience

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Author Spotlight: Understanding Microtubule Network in Drosophila Neuromuscular Junctions

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Using Drosophila Larval Neuromuscular Junction and Muscle Cells to Visualize Microtubule Network

Author Spotlight: Understanding Microtubule Network in Drosophila Neuromuscular Junctions

1,396 Views

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01:44 min

•

October 20, 2023

DOI:

10.3791/65774-v

DOI:

10.3791/65774-v

•

01:44 min

October 20, 2023

•

37 Views

Shengquan Zhang¹, Xiongxiong Wang¹, Zhihua Liu¹, Shan Jin¹, Chuan-Xi Mao¹

¹National & Local Joint Engineering Research Center of High-throughput Drug Screening Technology, School of life science,Hubei University

Transcript

Currently, microtubule dynamics are observed indirectly through microtubule-binding proteins such as EB1. Since alphabeta tubulin heterodimers are abundant in the cytoplasm, fold directly, neighboring tubulin heterodimers are lacking the dynamics of microtubule networks in vivo. Our protocol visualizes microtubules in neuromuscular synapses and muscle cells.

When combined with the powerful genetic tools available in Drosophila melanogaster, enabling a diverse range of phenotypic examinations and the genetic screening for the role of microtubule network regulatory proteins in the nervous system in vivo. Our research focuses on the relationship between the cytoskeleton and the neurodevelopment, as well as its implications for neurological diseases. We hope to discover the mechanism of neurodevelopment and the parthenogenesis for neurological diseases by highlighting the effect of cytoskeletal regulatory proteins on neurons.