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Detection of G1 Phase Single-Stranded DNA Foci via RPA2 Immunofluorescent Staining

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在细胞周期的 G1 期可视化单链 DNA 病灶

JoVE Journal
Biology

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JoVE Journal Biology

Visualizing Single-Stranded DNA Foci in the G1 Phase of the Cell Cycle

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在细胞周期的 G1 期可视化单链 DNA 病灶

Detection of G1 Phase Single-Stranded DNA Foci via RPA2 Immunofluorescent Staining

DOI:

10.3791/65926-v

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01:21 min

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December 22, 2023

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Qingyue Zhang², Marc A. Kerzhnerman², Nelson García-Vázquez^2,3, Gergely Rona^2,4,5

¹Department of Biochemistry and Molecular Pharmacology,NYU Grossman School of Medicine, ²The Laura and Isaac Perlmutter Cancer Center,NYU Langone Health, ³Department of Cell Biology,NYU Grossman School of Medicine, ⁴Howard Hughes Medical Institute,NYU Grossman School of Medicine, ⁵Institute of Enzymology, Centre of Excellence of the Hungarian Academy of Sciences,HUN-REN Research Centre for Natural Sciences

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Keywords: Single-stranded DNA DNA Damage Response DNA Repair RPA2 Cell Cycle G1 Phase Immunofluorescence DNA Lesions DNA Polymerases SsDNA Foci

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