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Extraction and Enzymatic Purification of Microglia from the Mouse Spinal Cord

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小鼠脊髓小胶质细胞的快速高效富集

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Rapid and Efficient Enrichment of Mouse Spinal Cord Microglia

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小鼠脊髓小胶质细胞的快速高效富集

Extraction and Enzymatic Purification of Microglia from the Mouse Spinal Cord

DOI:

10.3791/65961-v

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01:52 min

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September 22, 2023

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Claudia I Gutiérrez-Román², María E Meléndez Camargo, Cuauhtémoc C García Rojas, Miguel Jimenez Olvera, Sandra H Gutiérrez Román, Oscar Medina-Contreras

¹Epidemiology, Endocrinology & Nutrition,Mexico Children’s Hospital, ²Pharmacy Department, National School of Biological Sciences,National Polytechnic Institute, ³Graduate Department,Dr. Eduardo Liceaga General Hospital of Mexico, ⁴Pain Clinic and Palliative Care,Dr. Eduardo Liceaga General Hospital of Mexico, ⁵General Surgery Service,Metropolitan Angeles Hospital

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Keywords: Spinal Cord Microglia Purification Isolation Flow Cytometry CD11b CD45 Collagenase Density Gradient Heterogeneity Central Nervous System Neurological Pathologies Chronic Pain

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