Using <em>Ex Vivo</em> Live Imaging to Investigate Cell Divisions and Movements During Mouse Dental Renewal

Abinaya Sundari Thooyamani; Elias Shahin; Sanako Takano; Amnon Sharir; Jimmy  K. Hu

doi:10.3791/66020

Journal

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Developmental Biology

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Using Ex Vivo Live Imaging to Investigate Cell Divisions and Movements During Mouse Dental Renewal

JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology

Using Ex Vivo Live Imaging to Investigate Cell Divisions and Movements During Mouse Dental Renewal

Using Ex Vivo Live Imaging to Investigate Cell Divisions and Movements During Mouse Dental Renewal

1,238 Views

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07:37 min

•

October 27, 2023

DOI:

10.3791/66020-v

DOI:

10.3791/66020-v

•

07:37 min

October 27, 2023

•

6 Views

Abinaya Sundari Thooyamani*¹, Elias Shahin*², Sanako Takano¹, Amnon Sharir², Jimmy K. Hu^1,3

¹School of Dentistry,University of California Los Angeles, ²The Institute of Biomedical and Oral Research, Faculty of Dental Medicine,Hebrew University of Jerusalem, ³Molecular Biology Institute,University of California Los Angeles

Transcript

To maintain the correct cellular organization during organ renewal, tissue approaching the cells move and dividing specific orientations and patterns. It is therefore important to understand the rules and regulations of these dynamic cellular events. The mouse incisor is an emerging model for adult stem cell research, and many genetic tools are available in the system to study gene functions, and to label cells for lineage tracing.

Most studies to date use adult dental samples collected and fixed its specific time points, and this prevents us from examining life cell behavior. In this protocol, we describe a method to track lifestyles in the adult mouse incisor, and this technique will enable future studies that aim to understand how dynamic cell behaviors contribute to the renewal and repairment of dental tissues.

Summary

Ex vivo live imaging is a powerful technique for studying the dynamic processes of cellular movements and interactions in living tissues. Here, we present a protocol that implements two-photon microscopy to live track dental epithelial cells in cultured whole adult mouse incisors.