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Differentiation and Characterization of Osteoclasts from Human Induced Pluripotent Stem Cells
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Developmental Biology
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JoVE Journal Developmental Biology
Differentiation and Characterization of Osteoclasts from Human Induced Pluripotent Stem Cells

Differentiation and Characterization of Osteoclasts from Human Induced Pluripotent Stem Cells

DOI:
10.3791/66527-v

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10:52 min

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March 22, 2024

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, , , ,
1Department of Bioengineering, Department of Medicine,University of Washington, 2Department of Orthopedics and Trauma Surgery, Medical Faculty Mannheim,Heidelberg University

Chapters

  • 00:03Introduction
  • 01:16Passaging iPSCs
  • 03:32Embryoid Body Induction
  • 06:10Hematopoietic Differentiation
  • 08:31M-CSF Maturation and Osteoclast Differentiation
  • 09:25Representative Results
  • 10:19Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol presents the differentiation of human osteoclasts from induced pluripotent stem cells (iPSCs) and describes methods for the characterization of osteoclasts and osteoclast precursors.

Tags

Keywords: OsteoclastsHuman Induced Pluripotent Stem Cells (iPSCs)DifferentiationCharacterizationPassagingCD34+ Peripheral Blood Mononuclear CellsBone Disease ResearchCancer ResearchEndoprosthesis ResearchExpandabilityDMEM/F-12DispaseHEPESCell CultureCell Aggregates

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