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Modeling Osteosarcoma Using Li-Fraumeni Syndrome Patient-derived Induced Pluripotent Stem Cells
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1Department of Integrative Biology and Pharmacology, McGovern Medical School,The University of Texas Health Science Center at Houston, 2Graduate School of Biomedical Sciences,The University of Texas MD Anderson Cancer Center UTHealth, 3Department of Musculoskeletal Oncology,The First Affiliated Hospital of Sun Yat-sen University, 4Women’s Health Institute,Cleveland Clinic Foundation, 5Center for Stem Cell and Regenerative Medicine, The Brown Foundation Institute of Molecular Medicine for the Prevention of Human Diseases,The University of Texas Health Science Center at Houston, 6Center for Precision Health, School of Biomedical Informatics and School of Public Health,The University of Texas Health Science Center at Houston
Chapters
- 00:04Title
- 00:47Differentiation of Li-Fraumeni Syndrome (LFS) Patient-derived Induced Pluripotent Stem Cell (iPSC) to Mesenchymal Stem Cell (MSC) (Days 0 – 28)
- 02:19iPSC-derived MSC Maturation (Day 28 – 45) and Differentiation of LFS MSC to Osteoblasts (Day 0 – 24)
- 04:33Xenograft In Vivo Tumorigenesis Model Setup
- 06:39Results: Representative LFS MSC Differentiation
- 08:10Conclusion
Here, we present a protocol for the generation of induced pluripotent Stem Cells (iPSCs) from Li-Fraumeni Syndrome (LFS) patient derived fibroblasts, differentiation of iPSCs via mesenchymal stem cells (MSCs) to osteoblasts, and modeling in vivo tumorigenesis using LFS patient-derived osteoblasts.
