Establishment of Tumor Organoids, Carcinoma-Associated Fibroblasts, and Counterpart Fibroblasts from the Same Esophageal Cancer Patient

This protocol describes a method for simultaneously isolating cancer organoids, carcinoma-associated fibroblasts (CAFs), and non-tumor-associated fibroblasts (CFs) from the same patient with esophageal squamous cell carcinoma (ESCC). Employment of these patient-matched materials could avoid issues from inter-patient variability and provide an appropriate experimental platform for ESCC research.