Journal
/
Cancer Research
/
A Mouse Model to Investigate the Role of Cancer-Associated Fibroblasts in Tumor Growth
JoVE Journal
Cancer Research
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Cancer Research
A Mouse Model to Investigate the Role of Cancer-Associated Fibroblasts in Tumor Growth

A Mouse Model to Investigate the Role of Cancer-Associated Fibroblasts in Tumor Growth

DOI:
10.3791/61883-v

•

06:35 min

•

December 22, 2020

•

, , , , , , , , , , , , , , , , ,
1Department of Molecular, Cell and Developmental Biology,University of California, Los Angeles, 2Department of Biological Chemistry, David Geffen School of Medicine,University of California, Los Angeles, 3Department of Molecular Biology,Princeton University, 4Molecular Biology Institute,University of California, Los Angeles

Chapters

  • 00:05Introduction
  • 00:43Plating the Cancer Cells
  • 01:56Preparation of Cancer Cells and Fibroblasts for Injection into Mice
  • 04:13Inject Cancer Cells and Fibroblasts into Mice
  • 05:21Results: Analysis of Cancer-Associated Fibroblasts in Tumor Growth in Mice
  • 06:06Conclusion

Summary

Automatic Translation

Automatic Translation

A protocol to co-inject cancer cells and fibroblasts and monitor tumor growth over time is provided. This protocol can be used to understand the molecular basis for the role of fibroblasts as regulators of tumor growth.

Tags

Keywords: Cancer-associated FibroblastsTumor GrowthMelanomaMouse ModelCell CultureTrypsin-EDTAHemocytometerCell Counting

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/61883?article_type=v

Tumor Engraftment in a Xenograft Mouse Model of Human Mantle Cell Lymphoma
check_url/61883?article_type=v

A Method to Define the Effects of Environmental Enrichment on Colon Microbiome Biodiversity in a Mouse Colon Tumor Model
check_url/61883?article_type=v

Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer
check_url/61883?article_type=v

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
check_url/61883?article_type=v

A 3D Spheroid Model as a More Physiological System for Cancer-Associated Fibroblasts Differentiation and Invasion In Vitro Studies
check_url/61883?article_type=v

Isolation of Proximal Fluids to Investigate the Tumor Microenvironment of Pancreatic Adenocarcinoma
check_url/61883?article_type=v

Isolation of Primary Cancer-Associated Fibroblasts from a Syngeneic Murine Model of Breast Cancer for the Study of Targeted Nanoparticles
check_url/61883?article_type=v

An Ex Vivo Brain Slice Model to Study and Target Breast Cancer Brain Metastatic Tumor Growth
check_url/61883?article_type=v

A Three-Dimensional Spheroid Model to Investigate the Tumor-Stromal Interaction in Hepatocellular Carcinoma
check_url/61883?article_type=v

Molecular and Immunologic Techniques in a Genetically Engineered Mouse Model of Gastrointestinal Stromal Tumor

Read Article