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JoVE Journal
Biology

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Aortic Ring Assay
 

Aortic Ring Assay

Article DOI: 10.3791/1564-v 09:12 min November 24th, 2009
November 24th, 2009

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Summary

Angiogenesis, the sprouting of blood vessels from pre-existing vasculature, is associated with both natural and pathological processes. Here we demonstrate an aortic ring assay that allows angiogenic potentiators and inhibitors to be directly added to aortic rings in culture. Sprouting and neovessel outgrowth can be determined by inspecting the aortic rings over a period of 6-12 days.

Tags

Aortic Ring Assay Angiogenesis Blood Vessels Endothelial Cells Organ Culture Aorta Basement Matrix Extract (BME) Angiogenic Factors Inhibitors Of Angiogenesis Co-culture Paracrine Angiogenic Effects Sprouting Stereomicroscope Neovessel Outgrowth Phase Microscopy Supernatants Angiogenic Markers Anti-angiogenic Factors Cell Death Markers Nitrite
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