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Live Cell Imaging of Primary Rat Neonatal Cardiomyocytes Following Adenoviral and Lentiviral Transduction Using Confocal Spinning Disk Microscopy
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Live Cell Imaging of Primary Rat Neonatal Cardiomyocytes Following Adenoviral and Lentiviral Transduction Using Confocal Spinning Disk Microscopy

Article DOI: 10.3791/51666-v • 13:36 min • June 24th, 2014
June 24th, 2014 •
1,2, 2, 2, 2, 2, 2
1Max-Planck-Institute for Molecular Biomedicine and Institute of Cell Biology, 2Department of Internal Medicine, Yale Cardiovascular Research Center and Section of Cardiovascular Medicine

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This protocol describes a method of live cell imaging using primary rat neonatal cardiomyocytes following lentiviral and adenoviral transduction using confocal spinning disk microscopy. This enables detailed observations of cellular processes in living cardiomyocytes.

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Live Cell Imaging Primary Rat Neonatal Cardiomyocytes Adenoviral Transduction Lentiviral Transduction Confocal Spinning Disk Microscopy Timelapse Images Microscope Technology Cellular Events Phototoxicity Transduction Rate Expression Levels Autofocus System Exogenously Engineered Proteins SiRNAs Chemical Modulators
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