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Visualization of Cell Cycle Variations and Determination of Nucleation in Postnatal Cardiomyocytes
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Developmental Biology
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JoVE Journal Developmental Biology
Visualization of Cell Cycle Variations and Determination of Nucleation in Postnatal Cardiomyocytes

Visualization of Cell Cycle Variations and Determination of Nucleation in Postnatal Cardiomyocytes

DOI:
10.3791/55204-v

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09:41 min

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February 24, 2017

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, , , ,
1Institute of Physiology I,University of Bonn

Chapters

  • 00:05Title
  • 00:53Cardiomyocyte Isolation
  • 02:52Cardiomyocyte siRNA Transfection and Confocal Video Microscopy Analysis
  • 06:52Results: Representative Cardiomyocyte Analyses
  • 08:59Conclusion

Summary

Automatic Translation

Automatic Translation

To distinguish cell division from cell cycle variations in cardiomyocytes, we present protocols using two transgenic mouse lines: Myh6-H2B-mCh transgenic mice, for the unequivocal identification of cardiomyocyte nuclei, and CAG-eGFP-anillin mice, for distinguishing cell division from cell cycle variations.

Tags

Keywords: Cell CycleCardiomyocytesNucleationPostnatalVisualizationCell DivisionPloidyMulti-nucleationEGFPH2B-mCherryCell IsolationEnzyme DigestionCell CountingCell CultureSiRNA Transfection

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