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Journal / Developmental Biology / A Simple Chamber for Long-term Confocal Imaging…
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Developmental Biology

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JoVE Journal Developmental Biology
A Simple Chamber for Long-term Confocal Imaging of Root and Hypocotyl Development
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A Simple Chamber for Long-term Confocal Imaging of Root and Hypocotyl Development

Article DOI: 10.3791/55331-v • 07:59 min • May 17th, 2017
May 17th, 2017 •
1, 1
1Department of Plant Sciences, University of Oxford

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Presented here is a simple technique for high-resolution confocal time-lapse imaging of root and hypocotyl development for up to 3 days using high numerical-aperture objectives and perfluorodecalin as an immersion medium.

Tags

Confocal Imaging Root Development Hypocotyl Development Plant Morphogenesis Sub-cellular Resolution Confocal Fluorescence Microscopy Time Lapse Imaging Profusion Systems Perfluorodecalin Glass Cutter Cyanoacrylate Glue Double Sided Tape Glass Strips Slide Gasket Gas Permeable PDMS Gum Absolute Ethanol
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