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Quantitative Immunohistochemistry of the Cellular Microenvironment in Patient Glioblastoma Resections
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Quantitative Immunohistochemistry of the Cellular Microenvironment in Patient Glioblastoma Resections

Article DOI: 10.3791/56025-v • 05:45 min • July 31st, 2017
July 31st, 2017 •
1, 1
1Biomedical Engineering, University of Virginia

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This protocol was developed to quantitatively identify tumor microenvironment components in glioblastoma patient resections using chromogenic immunohistochemistry and ImageJ.

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Quantitative Immunohistochemistry Cellular Microenvironment Glioblastoma Resections Chromogenic Immunohistochemistry Image J Tumor Microenvironment Components Physiologically Relevant Ratios Of Cells Patient Samples Straightforward Analysis Complex Glioblastoma Tumor Microenvironment Chromogenic Development Tissue Saturation Washes Antigen Unmasking Solution Heat Mediated Antigen Retrieval Microwave Boiling Vessel
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