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Digital Spatial Profiling for Characterization of the Microenvironment in Adult-Type Diffusely Infiltrating Glioma
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Cancer Research
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JoVE Journal Cancer Research
Digital Spatial Profiling for Characterization of the Microenvironment in Adult-Type Diffusely Infiltrating Glioma

Digital Spatial Profiling for Characterization of the Microenvironment in Adult-Type Diffusely Infiltrating Glioma

DOI:
10.3791/63620-v

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09:17 min

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September 13, 2022

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, , , , ,
1Department of Neurology,Dartmouth-Hitchcock Medical Center, 2Department of Pathology and Laboratory Medicine,Dartmouth-Hitchcock Medical Center, 3Department of Neurosurgery,Dartmouth-Hitchcock Medical Center, 4Dartmouth Cancer Center,Dartmouth-Hitchcock Medical Center

Chapters

  • 00:04Introduction
  • 01:08Preparation of Slides and Semi‐Automated IHC System
  • 02:50Antibody Incubation and Nuclei Staining
  • 03:39Fluorescence Visualization, ROI Identification, and UV Photocleavage
  • 05:17Protein Readout and Data Analysis
  • 07:51Results: Digital Spatial Profiling on Glioblastoma Samples
  • 08:47Conclusion

Summary

Automatic Translation

Automatic Translation

Proteomic dysregulation plays an important role in the spread of diffusely infiltrating gliomas, but several relevant proteins remain unidentified. Digital spatial processing (DSP) offers an efficient, high-throughput approach for characterizing the differential expression of candidate proteins that may contribute to the invasion and migration of infiltrative gliomas.

Tags

Keywords: Digital Spatial ProfilingDSPProtein QuantificationMicroenvironmentAdult-type Diffusely Infiltrating GliomaMultiplexingRegions Of InterestOncologyTarget VariabilityMalignant GrowthInvasionImmunohistochemistryBlocking SolutionScanningHydrophobic Barrier

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