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Cancer Research

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JoVE Journal Cancer Research
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Evaluating the Differentiation Capacity of Mouse Prostate Epithelial Cells Using Organoid Culture

Article DOI: 10.3791/60223-v • 10:38 min • November 22nd, 2019
November 22nd, 2019 •
*1, *1, 2, 2, 2,3,4,5,6
1Molecular Biology Interdepartmental Program, University of California, Los Angeles, 2Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, 3Department of Urology, David Geffen School of Medicine, University of California, Los Angeles, 4Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, 5Jonsson Comprehensive Cancer Center, University of California, Los Angeles, 6Molecular Biology Institute, University of California, Los Angeles
* These authors contributed equally

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Summary
Automatic Translation

Mouse prostate organoids represent a promising context to evaluate mechanisms that regulate differentiation. This paper describes an improved approach to establish prostate organoids, and introduces methods to (1) collect protein lysate from organoids, and (2) fix and stain organoids for whole-mount confocal microscopy.

Tags

Differentiation Capacity Mouse Prostate Epithelial Cells Organoid Culture 3D Organoid System Physiologically Relevant Pharmacological Manipulations Genetic Manipulations In Vivo Approach Matrix Trial Room Temperature Pipette Liquid Ice Integrity Of The Ring Organoid Growth Changing Media Mouse Basal Cells Luminal Cells Cell Density Master Mixes Downstream Application
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