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Making Patch-pipettes and Sharp Electrodes with a Programmable Puller
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Making Patch-pipettes and Sharp Electrodes with a Programmable Puller

Article DOI: 10.3791/939-v • 05:30 min • October 8th, 2008
October 8th, 2008 •
1, 2, 2
1Department of Molecular and Cellular Physiology, Stanford University, 2Department of Molecular and Cellular Physiology, Stanford University School of Medicine

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Summary
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This video shows how to use a programmable puller to make patch pipettes and sharp electrodes for electrophysiology. The same procedure can be used to make a variety of glass tools, including injection needles.

Tags

Patch-pipettes Sharp Electrodes Glass Microelectrodes Programmable Puller Electrophysiology Capillary Glass Metal Filament Laser Gravity Motor Patch-clamp Recording Intracellular Recording Heat CRNA Injection Xenopus Oocytes Diameter Outer Diameter
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