Articles by Amulya M. Iyer in JoVE

Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Using the STEMCCA Lentiviral Vector

JoVE 4327 10/31/2012

Andreia Gianotti Sommer¹, Sarah S. Rozelle¹, Spencer Sullivan², Jason A. Mills³, Seon-Mi Park¹, Brenden W. Smith¹, Amulya M. Iyer¹, Deborah L. French³, Darrell N. Kotton¹, Paul Gadue³, George J. Murphy¹, Gustavo Mostoslavsky¹

¹Center for Regenerative Medicine (CReM), Boston University School of Medicine, ²Department of Hematology, Children's Hospital of Philadelphia, ³Center for Cellular and Molecular Therapeutics, Children's Hospital of Philadelphia

Here we show a simple and effective protocol for the generation of human iPSCs from 3-4 ml of peripheral blood using a single lentiviral reprogramming vector. Reprogramming of readily available blood cells promises to accelerate the utilization of iPSC technology by making it accessible to a broader research community.