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Medicine
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划痕迁移分析和背皮折叠室的体外和在Vivo分析伤口愈合
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Medicine
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JoVE Journal
Medicine
Scratch Migration Assay and Dorsal Skinfold Chamber for In Vitro and In Vivo Analysis of Wound Healing
Please note that all translations are automatically generated.
Click here for the English version.
划痕迁移分析和背皮折叠室的体外和在Vivo分析伤口愈合
DOI:
10.3791/59608-v
•
09:34 min
•
September 26, 2019
•
Anouar Belkacemi
,
Matthias W. Laschke
,
Michael D. Menger
,
Veit Flockerzi
1
Institute of Experimental and Clinical Pharmacology and Toxicology, Center for Molecular Signaling (PZMS)
,
Saarland University
,
2
Institute for Clinical and Experimental Surgery
,
Saarland University
Chapters
00:04
Title
01:05
Primary Cell Culture and Small Interfering RNA (siRNA) Transfection
02:56
In Vitro Wound Healing (Scratch Migration) Assay
04:13
In Vivo Skin Wound Healing Assay
07:11
Results: Representative In Vitro and In Vivo Wound Healing
08:43
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,我们提出了使用原纤维细胞进行体外划痕测定和小鼠体内皮肤伤口愈合测定的协议。这两种检测都是评估体外和体内伤口愈合的直截了当的方法。
Tags
Scratch Migration Assay
Dorsal Skinfold Chamber
Wound Healing
In Vitro
In Vivo
Cell Migration
Skin Wound
Pharmacologically Active Compounds
Fibroblasts
Genotype
Lipid-based Transfection Reagent
SiRNA
Cell Culture
Confluency
Scratch Wound
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