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Hemocompatibility Testing of Blood-Contacting Implants in a Flow Loop Model Mimicking Human Blood Flow
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Hemocompatibility Testing of Blood-Contacting Implants in a Flow Loop Model Mimicking Human Blood Flow

Hemocompatibility Testing of Blood-Contacting Implants in a Flow Loop Model Mimicking Human Blood Flow

DOI:
10.3791/60610-v

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09:41 min

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March 05, 2020

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, , , , , , , ,
1Department of Thoracic, Cardiac and Vascular Surgery,University Hospital Tuebingen, 2Acandis GmbH, 3Institute of Biomedical Engineering,University of Stuttgart, 4Institute of Macromolecular Chemistry,Academy of Sciences of the Czech Republic

Chapters

  • 00:05Introduction
  • 00:50Heparin-Loaded Monovette Preparation and Blood Sampling
  • 01:44Flow Loop Preparation
  • 02:24Hemocompatibility Testing
  • 02:51Whole Blood Count Analysis
  • 03:25Citrate Plasma Collection
  • 04:05EDTA and Citrate, Theophylline, Adenosine, and Dipyridamole (CTAD) Plasma Collection
  • 05:19Thrombin-Anti-Thrombin III (TAT) Quantification
  • 06:48Scanning Electron Microscopy (SEM) Sample Preparation
  • 07:30Results: Representative Hemocompatibility Testing
  • 09:02Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes a comprehensive hemocompatibility evaluation of blood-contacting devices using laser-cut neurovascular implants. A flow loop model with fresh, heparinized human blood is applied to mimic blood flow. After perfusion, various hematologic markers are analyzed and compared to the values gained directly after blood collection for hemocompatibility evaluation of the tested devices.

Tags

Hemocompatibility TestingBlood-contacting ImplantsFlow LoopHuman Blood FlowInternational Standard OrganizationThrombotic EventsAnticoagulantsBiomaterial ResearchHeparin LoadingNeurovascular Laser Cut ImplantPeristaltic PumpWhole Blood Count AnalysisEDTA

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