/
/
Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing (IEF) Method
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:04Introduction
- 00:45Setting Up IEF Unit
- 02:55IEF and Gymnema sylvestre
- 04:17IEF and C. albicans
- 06:45Gymnema sylvestre Bioactivity
- 07:36Results: Isolating Proteins, Peptides, and Small Molecules
- 09:21Conclusion
The objective is to fractionate and isolate bioactive small molecules, peptides from a complex plant extract, and proteins from pathogenic microbes by employing liquid-phase isoelectric focusing (IEF) method. Further, the separated molecules were identified and their bioactivity confirmed.
