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Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing (IEF) Method
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing (IEF) Method
DOI:

09:57 min

June 14, 2020

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Chapters

  • 00:04Introduction
  • 00:45Setting Up IEF Unit
  • 02:55IEF and Gymnema sylvestre
  • 04:17IEF and C. albicans
  • 06:45Gymnema sylvestre Bioactivity
  • 07:36Results: Isolating Proteins, Peptides, and Small Molecules
  • 09:21Conclusion

Summary

Automatic Translation

The objective is to fractionate and isolate bioactive small molecules, peptides from a complex plant extract, and proteins from pathogenic microbes by employing liquid-phase isoelectric focusing (IEF) method. Further, the separated molecules were identified and their bioactivity confirmed.

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