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Journal
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Biology
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Organotypic Slice Culture of E18 Rat Brains
JoVE Journal
Biology
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JoVE Journal
Biology
Organotypic Slice Culture of E18 Rat Brains
Organotypic Slice Culture of E18 Rat Brains
DOI:
10.3791/235-v
•
18:48 min
•
July 11, 2007
•
Laura Elias
,
Arnold Kriegstein
1
Institute for Regeneration Medicine
,
University of California, San Francisco – UCSF
Chapters
00:01
Title
00:16
Introduction
00:52
Tools and Reagents
01:45
Preparation
04:28
Brain Dissection
06:46
Embedding Brains in Agarose
08:57
Sectioning Brains
15:01
Preparing Brain Slices for Culture
18:08
Conclusion
18:31
End Credits
Tags
Organotypic Slice Culture
E18 Rat Brains
Brain Development
In-vivo System
Manipulations
Time-lapse Microscopy
Gene Expression
Ganglionic Emanances
In-utero Electroporation
Pharmacological Studies
Video Protocol
Rat Embryonic Day 18 Embryos
Dissecting Embryos
Low Melt Agarose
Vibratome
Plating Slices
Filters
Culture Dishes
Article
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