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Cerebrovascular Casting of the Adult Mouse for 3D Imaging and Morphological Analysis
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
Cerebrovascular Casting of the Adult Mouse for 3D Imaging and Morphological Analysis
DOI:

06:42 min

November 30, 2011

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Chapters

  • 00:05Title
  • 01:30Clearing Blood from the Vascular System
  • 02:43Perfusion of Casting Agent into the Vascular System
  • 04:02Collection and Processing of Tissue for Multiple Imaging Tools
  • 04:51Representative Images of Casted Vasculature using Multiple Imaging Tools
  • 05:57Conclusion

Summary

Automatic Translation

In this article, we present a simple, practical technique for cerebrovascular casting that is easy to perform and can be utilized to image the vascular tree of the adult mouse brain.

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