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PDMS Device Fabrication and Surface Modification

1, 1, 1

1Havard Medical School, MGH - Massachusetts General Hospital

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    Summary

    Date Published: 10/01/2007, Issue 8; doi: 10.3791/319

    Cite this Article

    Kotz, K., Cheng, X., Toner, M. PDMS Device Fabrication and Surface Modification. J. Vis. Exp. (8), e319, doi:10.3791/319 (2007).

    Disclosures

    The authors have nothing to disclose.

    Comments

    15 Comments

    I would like to know about the chaotic mixer for microchannels, could you please help me on that? Esmaeil.
    Reply

    Posted by: AnonymousFebruary 16, 2008, 2:22 AM

    What would you like to know?  We have a fair bit of experience in our lab with different mixer designs.  Is there a particular application that you looking into? Ken 
    Reply

    Posted by: AnonymousFebruary 18, 2008, 9:01 AM

    Hello David, The devices will soon be commercially available. Please email xonamicrofluidics@gmail.com for more information
    Reply

    Posted by: Joseph H.June 4, 2008, 7:51 PM

    during silanization of surface, you use 5% of silane is it in absolute ethanol or in H²O? did you characterize surface coverage of functional groups on channel surface?
    Reply

    Posted by: AnonymousJuly 4, 2008, 12:33 PM

    We do 5% silane in either absolute ethanol or in 95% ethanol (5% H²0).  Water catalyzes the silanization and so you need to react a bit longer with the anhydrous ethanol to get good coverage. Typically for anhydrous conditions, we react for ~30 minutes and for the 5% aqueous, we react only 10 minutes. We did not fully characterize the surface coverage of the functional groups on the surface.  You could take the device apart and use XPS to look at the atomic composition of the surface.  We determined the optimal conditions more empirically than that - we modified conditions for the highest density surface coverage by the cells that we wanted to capture.   Ken
    Reply

    Posted by: AnonymousJuly 7, 2008, 10:09 AM

    hi how can i download this video please ? my email address : sg_ghoreishi@sut.ac.ir
    Reply

    Posted by: AnonymousJuly 17, 2008, 5:04 AM

    Hi, Can you please tell me what was the pH of the silane solution that you used? Thanks in anticipation. Regards nidhi
    Reply

    Posted by: AnonymousAugust 4, 2008, 9:04 PM

    It was neutral pH, but can and perhaps should be slightly acidic.  You can make the ethanol solution slightly acidic ~pH 5 with acetic acid.  This will speed up the reaction, and you will need to shorten the time in silane/ethanol solution to about 5 minutes. Also be sure to wash throughly if you go this route.
    Reply

    Posted by: AnonymousAugust 5, 2008, 9:52 AM

    very ingenious device!Great!
    Reply

    Posted by: AnonymousOctober 25, 2008, 7:46 AM

    Great article - thanks for sharing.  I had one question.  Where did you get the spouts for pouring PDMS?  PDMS pouring can be such a mess, and it looks like you have found a nice solution.  If you could share the product information, I would appreciate it.  Thanks!  
    Reply

    Posted by: AnonymousNovember 10, 2008, 6:23 PM

    Dear Kotz, Great work!!! I have a question not related to the experiment. I wonder from where you got those dispensers for the PDMS and the curing agent. Do you get these from Dow Corning? Or you have any other supplier. I will appreciate if you can please share this information with me. My email ID is a.asthana@uq.edu.au. Best regards Amit  
    Reply

    Posted by: Amit A.April 14, 2009, 8:11 PM

    Dear Kenneth Kotz

    I have found your work as one of the best processes in microfluidic fabrication.
    I have tried to repeat this process in my laboratory, but I have a problem for peeling
    off PDMS from master mould. Would you please more explain for me about this process? The
    material of Petri dish that you used is glass or plastic? And when I pour PDMS on the
    master, during the curing process PDMS adhere to the Petri dish either glass and plastic,
    in addition the adhesive problem related PDMS and master mould.
    I want to explain more about my problem. In fact I used glass Petri dish and put the
    master mould in it and when I pour PDMS on the master mold, it will be spread and some of
    the PDMS penetrate under the master mould and causes the adhesion between glass wall of
    the Petri Dish and master mould and as a result the master mould break when I want to
    peel off PDMS and pick up mould master.
    In the other hand although I use silanization process for peeling off the
    PDMS from master mould, it is not effective. I think these problems are related together.
    Would you please explain for me how can I solve them?
    I would appreciate you for helping me.
    I am looking forward to hearing from you.

    Thanks
    H.Madadi
    Reply

    Posted by: AnonymousJune 28, 2011, 5:16 AM

    The master mold was fixed in a polystyrene petri dish with clean room adhesive tape taping the edge. A small amount of liquid PDMS dŒs penetrate under the wafer, but we cut out the PDMS device with a surgical razor blade. If you want to release PDMS from the whole wafer, you need to cut along the edge of the wafer first using a razor blade.
    Reply

    Posted by: AnonymousJune 28, 2011, 10:19 AM

    Hello,
    I am currently about to start a project investigating glycoproteins-E-selectin in ovarian cancer, and will need to use a microfluidic device. This device is being fabricated by the mechanical engineering department in my university.
    However, they have minimal experience in this area, and have asked me to research what the microfluidic channel would be coated with for our purposes.
    I would eventually need to be able to both immobilise proteins on the walls of the channel and make cells adhere in a monolayer in the channel. I am unable to find much clear information whether a silane coating will fulfil both these functions, and am looking for more information.
    Could you please tell me about what sort of coating will be required in order to be able to achieve both these aims?
    Thank you,
    --
    Shashwati Kala
    Reply

    Posted by: Shashwati K.January 29, 2013, 3:58 AM

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