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Immunology and Infection

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JoVE Journal Immunology and Infection
Real-time Imaging of Myeloid Cells Dynamics in ApcMin/+ Intestinal Tumors by Spinning Disk Confocal Microscopy
An Optimized Protocol to Analyze Glycolysis and Mitochondrial Respiration in Lymphocytes
Journal (Immunology and Infection)
An Optimized Protocol to Analyze Glycolysis and Mitochondrial Respiration in Lymphocytes
Intravital Microscopy of the Inguinal Lymph Node
Journal (Immunology and Infection)
Intravital Microscopy of the Inguinal Lymph Node
 

Real-time Imaging of Myeloid Cells Dynamics in ApcMin/+ Intestinal Tumors by Spinning Disk Confocal Microscopy

Article DOI: 10.3791/51916-v • 05:21 min • October 6th, 2014
October 6th, 2014 •
*1,2, *2, 2
1Department of Oncology, INSERM U661, Functional Genomic Institute, 2Department of Anatomy, University of California
* These authors contributed equally

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Summary
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By using transgenic reporter mice and injectable fluorescent labels, long-term intravital spinning disk confocal microscopy enables direct visualization of myeloid cell behavior into intestinal adenoma in the ApcMin/+ colorectal cancer model.

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Real-time Imaging Myeloid Cells Dynamics ApcMin/+ Intestinal Tumors Spinning Disk Confocal Microscopy Intravital Imaging Techniques Live Cellular Behavior Organ And Tumor Accessibility Intestine Surgical Procedure Transgenic Fluorescent Reporter Mice Injectable Tracers Or Antibodies Four-color Multi-region Micro-lensed Spinning Disk Confocal Microscope Long-term Continuous Imaging Rapid Image Acquisition ApcMin/+ Mice Multiple Adenomas Small Intestine C-fms-EGFP Mice ACTB-ECFP Mice Intestinal Epithelial Cells Crypts Labeling Tumor Components Blood Vessels Neutrophils Positioning The Tumor For Imaging Serosal Surface Time-lapse Movies
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