Golden Shiners
Golden Shiners - Related Videos
06:12
Production of Genetically Engineered Golden Syrian Hamsters by Pronuclear Injection of the CRISPR/Cas9 Complex
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Cited by 24 •
2018
Pronuclear (PN) injection of the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein-9 nuclease (CRISPR/Cas9) system is a highly efficient method for producing genetically engineered golden Syrian hamsters. Herein, we describe the detailed PN injection protocol for the production of gene knockout hamsters with the CRISPR/Cas9 system.
09:51
Rapid Verification of Terminators Using the pGR-Blue Plasmid and Golden Gate Assembly
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Cited by 2 •
2016
This protocol utilizes Golden Gate Assembly and the plasmid pGR-blue to rapidly quantify the strength of terminators found in silico.
10:08
The Golden Apple Snail Pomacea canaliculata: From Zygotes to Stable Mutant Lines
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Cited by 1 •
2025
This study developed a workflow for the generation and maintenance of stable mutant lines for the freshwater apple snail, Pomacea canaliculata. The protocols include the collection of zygotes, their microinjection with the CRISPR/Cas9 system components to induce genome editing, ex ovo culture of embryos, and genotyping after controlled crosses.
08:31
Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning
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Cited by 11 •
2021
The goal of this protocol is to provide a detailed, step-by-step guide for assembling multi-gene constructs using the modular cloning system based on Golden Gate cloning. It also gives recommendations on critical steps to ensure optimal assembly based on our experiences.
11:51
Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence
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Cited by 7 •
2018
Synthetic biology enables the engineering of proteins with unprecedented properties using the co-translational insertion of non-canonical amino acids. Here, we presented how a spectrally red-shifted variant of a GFP-type fluorophore with novel fluorescence spectroscopic properties, termed "gold" fluorescent protein (GdFP), is produced in E. coli via selective pressure incorporation (SPI).
FAQs
Golden shiners combine visual information with mechanosensory cues, allowing them to respond to nearby fish and changes in their surroundings. These inputs can influence swimming direction, spacing, movement, and feeding. Considering both sensory channels helps explain why behavioral responses emerge from interactions among individuals rather than from a single environmental signal.
Spacing provides a measurable link between individual decisions and group organization. As Golden shiners respond to nearby fish, they adjust their positions and movements, producing coordinated schooling patterns. Researchers can therefore examine how local responses scale into collective behavior, including social coordination and group-level changes when food, water conditions, or threats vary.
Food availability can alter feeding and movement, whereas a perceived threat can change swimming and spacing as fish respond to possible danger. Water conditions may further modify these behaviors. Comparing responses across these factors helps distinguish how Golden shiners balance feeding opportunities, social coordination, and predator avoidance within changing environments.
Researchers can track changes in swimming, spacing, movement, and feeding while varying environmental conditions or the presence of perceived threats. Observations at both individual and group levels reveal whether responses remain localized or become coordinated. This approach connects measurable behavior with broader questions about collective decision-making and predator-prey interactions.
Golden shiners support studies of behavioral ecology and fisheries research because their responses link individual actions with group behavior in aquatic environments. Investigators can use their schooling, foraging, and predator-avoidance responses to examine how environmental conditions shape animals and how social coordination affects interactions within a fish group.
Their behavior helps researchers investigate how groups make decisions, how individuals coordinate movement, and how predator-prey interactions unfold. Because Golden shiners respond to food availability, water conditions, nearby fish, and perceived threats, they also provide a way to study how environmental change influences both individual activity and collective organization in aquatic ecosystems.