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JoVE Journal
Bioengineering

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Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning
 

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning

Article DOI: 10.3791/61993-v
February 5th, 2021

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Summary February 5th, 2021

The goal of this protocol is to provide a detailed, step-by-step guide for assembling multi-gene constructs using the modular cloning system based on Golden Gate cloning. It also gives recommendations on critical steps to ensure optimal assembly based on our experiences.

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