Ciliated Epithelial Cells
Ciliated Epithelial Cells - Related Videos
11:46
Ex Vivo Culture of Primary Human Fallopian Tube Epithelial Cells
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Cited by 29 •
2011
The fallopian tube (FT) is emerging as an alternative site of origin for serous ovarian carcinoma (SOC). This protocol describes a novel method for the isolation and ex vivo culture of fallopian tube epithelial cells. This system recapitulates the in vivo epithelium and allows the study of SOC pathogenesis.
14:32
Primary Human Bronchial Epithelial Cells Grown from Explants
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Cited by 19 •
2010
Here we describe a detailed method for growing primary human bronchial epithelial cells from explants of human bronchial airway tissue including differentiated growth on an air-liquid interface. This method provides an abundant source of primary cells for investigating the role of the airway epithelium in human lung health and disease.
08:42
Immunostaining Phospho-epitopes in Ciliated Organs of Whole Mount Zebrafish Embryos
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Cited by 3 •
2016
Techniques are described to immunostain phospho-epitopes in whole zebrafish embryos and then conduct two-color fluorescent confocal localization in cellular structures as small as primary cilia. The techniques for fixing and imaging can define the location and kinetics of the appearance or activation of specific proteins.
10:38
Culturing of Human Nasal Epithelial Cells at the Air Liquid Interface
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Cited by 135 •
2013
Nasal epithelial cells, obtained through superficial scrape biopsy of human volunteers, are expanded and transferred onto tissue culture inserts. Upon reaching confluency, cells are grown at air liquid interface, yielding cultures of ciliated and non-ciliated cells. Differentiated nasal epithelial cell cultures provide viable experimental models for studying the respiratory mucosa.
11:52
Whole-cell Patch-clamp Recordings of Isolated Primary Epithelial Cells from the Epididymis
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Cited by 1 •
2017
We present a protocol that combines cell isolation and whole-cell patch-clamp recording to measure the electrical properties of the primary dissociated epithelial cells from the rat cauda epididymides. This protocol allows for investigation of the functional properties of primary epididymal epithelial cells to further elucidate the physiological role of the epididymis.
FAQs
Directional transport depends on the coordinated, rhythmic activity of many motile cilia, not merely on the presence of individual hairlike structures. Their collective beating creates consistent movement of fluid or material across the epithelial surface. This organization allows a tissue to perform surface transport while preserving the epithelial layer as a continuous, organized interface.
Within each cilium, the axoneme provides a microtubule-based structural framework, while dynein motor proteins power ciliary motion. This division of roles links architecture to activity: microtubules form the movement-supporting apparatus, and dynein supplies the motor function needed for beating. Examining both components helps explain how cellular structure generates tissue-level transport.
Cell junctions are important because ciliary transport must occur across an intact epithelial sheet. They connect neighboring cells and help maintain a continuous barrier while apical cilia move material along the surface. This combination prevents transport activity from being separated from tissue organization, making these cells useful for studying how barrier integrity and directional movement coexist.
In airway lining, the transported material is mucus containing trapped particles. Ciliary activity moves this material toward the throat, providing the cellular basis of mucociliary clearance. Studying this setting connects ciliary motion with a recognizable tissue function: coordinated activity at the cell surface helps clear material from the airways rather than leaving it on the epithelial lining.
Their role in the oviduct illustrates that cilia are not restricted to airway clearance. There, ciliary activity contributes to fluid movement, showing how the same motile apparatus can support transport in a different organ. Comparing these locations helps biologists relate a shared cellular mechanism to distinct tissue-level functions.
When ciliary motion is impaired, the coordinated transport normally produced at epithelial surfaces is disrupted. In airway tissue, that disturbance is directly relevant to mucociliary clearance and to diseases caused by defective ciliary movement. Studying axonemes, dynein motors, and epithelial organization therefore helps connect cellular structure and motion with failures of tissue-level transport.