Dsrna Delivery
Dsrna Delivery - Related Videos
02:37
Detection of Zika Viral dsRNA Replication Intermediates Using Immunocytochemistry
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2026
Source: Contreras, D., et al. Zika Virus Infectious Cell Culture System and the In Vitro Prophylactic Effect of Interferons. J. Vis. Exp. (2016)The video demonstrates the detection of Zika viral dsRNA intermediates in infected epithelial cells using immunocytochemistry. Infected cells are fixed and then blocked with a blocking solution. The primary and secondary antibodies are added. The nuclei are stained with a fluorescent dye. The labeled viral dsRNA is then seen under a fluorescence...
05:36
Larval RNA Interference in Silkworm Bombyx mori through Chitosan/dsRNA Nanoparticle Delivery
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Cited by 4 •
2024
Presented here is a protocol for chitosan/dsRNA nanoparticle delivery in silkworm Bombyx mori larvae to induce gene silencing through ingestion.
03:24
RNAi Plating for C. elegans Feeding: A Technique to Induce Target dsRNA Expression in E. coli
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2023
This video describes the principles behind RNAi treatment in C. elegans and demonstrates a protocol to knockdown lin-35 in a transgenic worm strain.
04:24
Injection of dsRNA into Female A. aegypti Mosquitos
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Cited by 8 •
2007
Reverse genetic approaches have proven extremely useful for determining which genes underly resistance to vector pathogens in mosquitoes. This video protocol illustrates a method used by the James lab to inject dsRNA into female A. aegypti mosquitoes, which harbor the dengue virus. The technique for calibrating injection needles, manipulating the injection setup, and injecting dsRNA into the thorax is illustrated.
08:41
Reproducible dsRNA Microinjection and Oviposition Bioassay in Mosquitoes and House Flies
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Cited by 5 •
2018
This protocol describes a microinjection methodology that we have standardized and used for several years to deliver specific quantities of nucleic acids directly to the hemolymph of mosquitoes and house flies. This protocol results in minimal injection mortality and allows dose correlated measurements of fecundity.
FAQs
Once inside a cell, delivered dsRNA can be processed by Dicer into small interfering RNAs. These molecules load into the RNA-induced silencing complex, which uses their sequence information to recognize complementary messenger RNA and promote its degradation. The resulting reduction in messenger RNA provides a molecular route for investigating how a selected gene contributes to cellular biology.
The cellular response depends on how the introduced dsRNA interacts with intracellular pathways. In some cases, Dicer processing leads to small interfering RNA production and sequence-specific messenger RNA degradation. In others, dsRNA activates RNA-sensing pathways. This distinction matters because an experiment may be designed either to examine a gene-specific effect or to study cellular responses to dsRNA itself.
Protection is important because delivery systems are used to help preserve dsRNA during transport and support its entry into target cells. Without an effective delivery approach, the intended RNA may not reach the relevant tissue or intracellular environment. Delivery design also helps researchers influence distribution, making it a central variable when interpreting gene-expression or RNA-sensing outcomes.
Delivery methods can affect where dsRNA travels by helping control its distribution across cells or tissues. They may also influence which target cells take up the RNA and whether it reaches the intracellular setting required for downstream activity. Consequently, delivery is not merely a transport step; it helps determine the biological location, extent, and interpretability of the response.
A typical workflow considers the delivery approach, protection of the dsRNA, and transport into the selected cells or tissue. After uptake, investigators assess whether the RNA is processed into small interfering RNAs, whether complementary messenger RNA is degraded, or whether RNA-sensing pathways are activated. These linked stages connect the delivery method to the observed biological outcome.
Researchers apply dsRNA delivery in gene-function studies and functional genomics to examine the consequences of changing expression of selected genes. It also supports disease modeling and the development of RNA-based strategies for research, therapeutics, and agriculture. The same general approach can therefore connect molecular investigations with broader studies of biological function and potential practical applications.