Ecotropic Virus
Ecotropic Virus - Related Videos
14:03
Transduction of Human Cells with Polymer-complexed Ecotropic Lentivirus for Enhanced Biosafety
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Cited by 8 •
2011
Lentiviruses are a valuable research tool for exploring gene function; however, researchers may wish to avoid production of pantropic lentivirus encoding known or suspected oncogenes. As an alternative, we present a safer protocol for use of ecotropic lentivirus on human cells modified to express the ecotropic receptor mSlc7a1.
07:35
Rescue and Characterization of Recombinant Virus from a New World Zika Virus Infectious Clone
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Cited by 13 •
2017
This protocol describes the recovery of infectious Zika virus from a two-plasmid infectious cDNA clone.
18:10
Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency
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Cited by 40 •
2011
The present article describes the steps required to isolate and characterize RNA polymerase fidelity variants of RNA viruses and how to use mutation frequency data to confirm fidelity changes in tissue culture.
09:08
Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus
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2021
For in-depth mechanistic analysis of the respiratory syncytial virus (RSV) RNA synthesis, we report a protocol of utilizing the chaperone phosphoprotein (P) for coexpression of the RNA-free nucleoprotein (N0) for subsequent in vitro assembly of the virus-specific nucleocapsids (NCs).
03:43
Virus Plaque Assay: A Technique to Measure Infectious Herpes Simplex Virus via Quantification of Plaques Formed in Monolayer of Virus-Infected Cells
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2025
This video describes the virus plaque assay of the infectious herpes simplex virus or HSV. The diluted viral particles are inoculated to the monolayer of epithelial cells. Upon incubation, the virus forms plaques and is counted visually to determine the infectious virus titer.
FAQs
Specificity begins at the cell surface: envelope proteins recognize and bind the mCAT-1 receptor on mouse cells. This interaction helps the viral membrane enter the target cell, so receptor availability becomes a major determinant of whether infection can proceed. Studying this binding step allows researchers to connect molecular receptor recognition with the species-restricted tropism observed in murine systems.
After entry, the viral RNA serves as the template for reverse transcription, which produces DNA. That DNA can integrate into the host genome, linking an early entry event to a potentially persistent genetic state in the infected cell. This sequence is important for analyzing retroviral replication and for understanding why infection can support stable expression of introduced genetic material.
Ecotropic and broader-host-range viral systems differ primarily in the cells their envelope-receptor interaction can support. In an ecotropic system, dependence on a receptor associated with compatible mouse cells creates a useful biological restriction rather than unrestricted cell access. Researchers can therefore examine host barriers and receptor specificity by relating successful infection to cell type and species compatibility.
An ecotropic retroviral experiment begins by choosing cells compatible with the system, then exposing them to the viral system under controlled laboratory conditions. The key experimental readout is whether infection occurs and whether introduced genetic material remains expressed. This setup lets investigators separate compatibility at the entry stage from downstream outcomes such as genome integration and stable expression.
These systems are useful when researchers need controlled delivery of introduced genetic material rather than only a model of viral infection. Successful transfer can produce stable expression in compatible cells because the retroviral DNA integrates into the host genome. Consequently, experiments can assess gene-transfer outcomes, viral replication, and the influence of host-cell properties on expression.
In biology, the restricted host range provides a practical way to investigate host barriers. Researchers can compare cells according to whether they present the relevant receptor and support the subsequent intracellular steps. The resulting pattern of infection helps connect species specificity with molecular entry, reverse transcription, and integration, making ecotropic systems informative models for virus-host interactions.