Hard Palate Harvest

Hard palate harvest is a surgical technique for collecting tissue from the roof of the mouth, where the dense, keratinized mucosa can serve as a biological graft. The procedure typically involves exposing the hard palate, making controlled incisions, and separating a mucoperiosteal or connective-tissue segment while preserving the surrounding structures and supporting hemostasis. Harvested palatal tissue is used in periodontal and oral reconstructive procedures to increase soft-tissue thickness, cover exposed root surfaces, and repair localized defects. In biology and translational research, the technique also provides a model for studying wound healing, epithelial regeneration, tissue remodeling, and donor-site recovery.

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Research

JoVE EoE - Rodent Models

Dissection of Palate Tissue from Adult Murine Model: A Surgical Procedure to Harvest Hard Palate and Soft Palate from Oral Cavity of Adult Mouse

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2025

This video demonstrates a surgical procedure to dissect the palate tissue from a murine model.

Education

JoVE Science Education - Engineering

Rockwell Hardness Test and the Effect of Treatment on Steel

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2023

Source: Roberto Leon, Department of Civil and Environmental Engineering, Virginia Tech, Blacksburg, VA Hardness testing is one of the most universally valuable mechanical tests available to engineers, as it is both simple and relatively inexpensive for the wealth of information and data it produces. Hardness testing, generally in the form of a surface penetration test, is both quicker and less destructive than tensile testing. Hardness provides a linear relationship with tensile strength over a...

Live Imaging of Mouse Secondary Palate Fusion

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Cited by 11 •

2017

Here, we present a protocol for live imaging of mouse secondary palate fusion using confocal microscopy. This protocol can be used in combination with a variety of fluorescent reporter mouse lines, and with pathway inhibitors for mechanistic insight. This protocol can be adapted for live imaging in other developmental systems.

Method of Studying Palatal Fusion using Static Organ Culture

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Cited by 6 •

2015

Studies of palate development are motivated by the incidence of cleft palate, a birth defect that imposes a tremendous health burden and can leave lasting disfigurement. We demonstrate here a technique to culture palatal shelves that can be used to study different signaling pathways involved in palatal development and fusion.

Quantitative Hardness Measurement by Instrumented AFM-indentation

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Cited by 6 •

2016

This experimental protocol describes how atomic force microscopy can be used to measure hardness at the true nanometer scale and to detect single atomistic plasticity events.

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