Human Myocardium
Human Myocardium - Related Videos
12:31
In Vivo Modeling of the Morbid Human Genome using Danio rerio
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Cited by 66 •
2013
Here, we present a systematic approach for developing physiologically relevant, sensitive and specific in vivo assays for interpreting variation in human pathology. Transient genetic manipulation via microinjection of WT and mutant human mRNA and morpholino (MO) antisense oligonucleotides harness the tractability of the developing zebrafish embryo to rapidly assay pathogenic mutations, especially, but not exclusively, in the context of human developmental disorders.
10:22
Enrichment of Bruch's Membrane from Human Donor Eyes
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Cited by 14 •
2015
Identifying proteins specifically associated with Bruch’s membrane in human eyes is an important step in understanding the biochemical mechanisms behind eye diseases such as age-related macular degeneration. This protocol describes how to enrich this sheet of extracellular matrix for down-stream biochemical analysis.
07:38
Techniques for Imaging Ca2+ Signaling in Human Sperm
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Cited by 17 •
2010
Stimulus-evoked [Ca2+]i signals of individual human sperm are assessed. Motile cells are loaded with Ca2+-sensitive fluorescent dye (AM-ester method) and immobilised in a perfusable chamber. Cells are imaged by time-lapse fluorescence microscopy and stimulated via the perfusing medium. Responses of single cells (or regions) are analysed offline using Excel.
14:03
Transduction of Human Cells with Polymer-complexed Ecotropic Lentivirus for Enhanced Biosafety
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Cited by 8 •
2011
Lentiviruses are a valuable research tool for exploring gene function; however, researchers may wish to avoid production of pantropic lentivirus encoding known or suspected oncogenes. As an alternative, we present a safer protocol for use of ecotropic lentivirus on human cells modified to express the ecotropic receptor mSlc7a1.
08:35
Human Pancreatic Islet Isolation: Part II: Purification and Culture of Human Islets
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Cited by 27 •
2009
Achieving high quality and appropriate quantity of human islets is one of the prominent prerequisites for successful islet transplantation. In this video, we describe step by step the procedures for human pancreatic islet isolation (part II: purification and culture of human islets) using a modified automated method.
FAQs
Electrical stimulation of cardiomyocytes triggers the release of calcium inside the cells. Calcium then enables interactions between actin and myosin, the contractile proteins arranged within sarcomeres. These interactions generate force during contraction, linking electrical activity to the mechanical work required for coordinated cardiac pumping.
Specialized connections between myocardial cells help electrical signals spread through the heart rather than remaining confined to individual cardiomyocytes. This coordination allows groups of cells to contract in a controlled rhythm. Studying these connections helps explain how cardiac electrical conduction supports organized activity across the heart chambers.
Sarcomeres provide the structural setting where actin and myosin interact after calcium is released in response to electrical signals. Their activity converts intracellular signaling into cardiomyocyte contraction and force generation. Examining sarcomeres therefore connects cellular-level events with the myocardium’s ability to support the heart’s pumping action.
Myocardial research examines how cardiac muscle tissue contributes to heart formation and how electrical signals move through cardiomyocytes and specialized cell connections. These areas reveal how structure, excitation, and coordinated contraction are related. The resulting biological context supports investigation of normal cardiac function as well as changes associated with disease.
Research on human myocardium helps examine how disruption or damage to cardiac muscle relates to myocardial infarction and heart failure. By connecting myocardial structure and activity with these disorders, investigators can study impaired cardiac function and evaluate therapeutic strategies intended to restore or protect the heart’s performance.
Myocardial studies support cardiotoxicity research, which examines harmful effects on cardiac tissue, as well as regenerative medicine aimed at restoring damaged function. The same knowledge contributes to engineered heart tissue and therapies designed to protect or improve cardiac performance, linking basic biological mechanisms with potential treatment development.