Induced Pluripotent Stem Cells
Induced Pluripotent Stem Cells - Related Videos
01:13
Induced Pluripotent Stem Cells
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2019
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
10:24
Transfecting and Nucleofecting Human Induced Pluripotent Stem Cells
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Cited by 26 •
2011
Despite recent advancements in genetic modification, transfection of human embryonic stem cells (HESCs) remains a capricious process. To our knowledge, systematic and efficient methods to transfect human induced pluripotent stem cells (iPSCs) have not been reported. Here, we describe robust protocols to efficiently transfect and nucleofect human iPSCs.
11:56
Generation of Mice Derived from Induced Pluripotent Stem Cells
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Cited by 16 •
2012
Generating induced pluripotent stem cell (iPSC) lines produces lines of differing developmental potential even when they pass standard tests for pluripotency. Here we describe a protocol to produce mice derived entirely from iPSCs, which defines the iPSC lines as possessing full pluripotency1.
08:25
Reprogramming Human Somatic Cells into Induced Pluripotent Stem Cells (iPSCs) Using Retroviral Vector with GFP
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Cited by 6 •
2012
A method to generate human induced pluripotent stem cells (iPSCs) via retrovirus-mediated ectopic expression of OCT4, SOX2, KLF4 and MYC is described. A practical way to identify human iPSC colonies based on GFP expression is also discussed.
04:22
Differentiation of Induced Pluripotent Stem Cells into Neural Progenitor Cells
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2025
The video demonstrates a technique for differentiating human induced pluripotent stem cells (hiPSCs) into neural progenitor cells (NPCs). Initially, the hiPSCs are cultured on a feeder layer of mouse embryonic fibroblasts (MEFs). Subsequently, the cells are detached and transferred to a biopolymer-coated plate to allow the MEFs to adhere to and separate from the hiPSCs. The suspended hiPSCs are then transferred to a V-bottom plate and centrifuged to form aggregates. Finally, the addition of a...
FAQs
Reprogramming factors alter the cell’s gene expression so its existing developmental identity is reset. This change moves a mature, specialized cell toward a stem-like state rather than simply expanding the original cell type. The mechanism matters because it provides a biological route for producing cells with broader developmental potential from adult tissue.
Skin and blood cells are adult somatic cells that can be used as accessible starting materials for reprogramming. Introducing the appropriate factors changes their developmental state, allowing researchers to derive patient-linked stem-like cells from mature tissue. This connection supports individualized biological models while avoiding the need to begin with embryonic tissue.
Returning a specialized cell to a pluripotent state changes its developmental program, so researchers must consider whether the resulting cells remain genetically stable and suitable for their intended use. These concerns are especially relevant to regenerative medicine, where safety influences whether laboratory-generated cells can be investigated as potential therapeutic material.
A typical workflow begins with an adult somatic cell, such as a skin or blood cell, followed by introduction of reprogramming factors. These factors alter gene expression and reset the cell’s developmental identity, producing cells in a pluripotent, stem-like state. Researchers can then study or apply the resulting cells in biological investigations.
Because they can be generated from a particular individual’s cells, iPSCs provide patient-specific systems for examining development and disease. Researchers can investigate how cellular identity changes and use these models to study disease-related biology in a controlled research setting. The patient connection can make observations more relevant to personalized research questions.
iPSCs can supply patient-specific cellular models for testing drug responses, helping researchers examine how cells associated with an individual respond in laboratory studies. Their ability to produce many body cell types also makes them relevant to regenerative medicine research. In both settings, genetic stability and safety remain important factors when interpreting results.