Living Bacterial Cells

Living bacterial cells are single-celled organisms that maintain metabolism, regulate their internal environment, respond to stimuli, and reproduce independently, making them fundamental to biology and ecosystems. Their plasma membrane controls exchange with the surroundings, while genetic information directs protein production and cellular processes; under suitable conditions, many bacteria grow by duplicating their DNA and dividing through binary fission. Studying living bacterial cells reveals how microbes adapt, communicate, and interact with hosts, nutrients, and environments. These cells support research in genetics, microbiology, biotechnology, infectious disease, environmental science, and the development of antimicrobial strategies.

Living Bacterial Cells - Related Videos

Research

JoVE Journal - Immunology and Infection

Visualization of Bacterial Toxin Induced Responses Using Live Cell Fluorescence Microscopy

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Cited by 15 •

2012

Methods for purifying the cholesterol binding toxin streptolysin O from recombinant E. coli and visualization of toxin binding to live eukaryotic cells are described. Localized delivery of toxin induces rapid and complex changes in targeted cells revealing novel aspects of toxin biology.

Research

JoVE Journal - Immunology and Infection
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Assessing Stomatal Response to Live Bacterial Cells using Whole Leaf Imaging

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Cited by 38 •

2010

We have developed a simple and reproducible protocol to access stomatal response to live bacteria. This method minimizes wounding and manipulation of the leaf as compared to the use of epidermal peels reported previously.

Visualizing Protein-DNA Interactions in Live Bacterial Cells Using Photoactivated Single-molecule Tracking

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Cited by 38 •

2014

Photoactivated localization microscopy (PALM) combined with single-molecule tracking allows direct observation and quantification of protein-DNA interactions in live Escherichia coli cells.

Preparation of a Microfluidic Plate with Bacterial Cells

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2025

Source: Sutlief, A. L., et al. Live Cell Analysis of Shear Stress on Pseudomonas aeruginosa Using an Automated Higher-Throughput Microfluidic System. J. Vis. Exp. (2019).This video demonstrates the preparation of a microfluidic plate for shear stress studies in bacterial cultures. After priming the channels with media, bacterial cells are introduced into the experimental channel and incubated for attachment. Residual media and culture are removed to prepare the system for subsequent shear...

Research

JoVE Journal - Biology
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Electron Cryotomography of Bacterial Cells

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Cited by 21 •

2010

We illustrate here how to use electron cryotomography (ECT) to study the ultrastructure of bacterial cells in near-native states, to "macromolecular" (~4 nm) resolution.

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